Cat.No. : LV00940Z
Titer: ≥1*10^7 TU/mL / ≥1*10^8 TU/mL / ≥1*10^9 TU/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | LV00940Z |
| Target Gene | lin28 |
| Product Type | Lentiviral particle |
| Titer | Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc. |
| Size | Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. | |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
Lin28 is a conserved RNA-binding protein in higher eukaryotes that regulates several important cellular functions related to development, glucose metabolism, differentiation, and pluripotency. Conditional reactivation of the Lin28 gene in adult mice significantly accelerates the wound healing process of injured fingers. This study aimed to investigate the effects of Lin28 gene overexpression on the proliferation of human dental pulp cells (HDPCs) and its mechanisms. The results showed that Lin28 promoted cell proliferation and the S-G2/M transition of HDPCs and directly bound to a set of cell cycle regulatory mRNAs in HDPCs. Through bioinformatics analysis and luciferase assays, the researchers confirmed that let-7a targets IGF2BP2. Silencing IGF2BP2 showed similar cellular and molecular effects as let-7a. Similarly, restoring IGF2BP2 counteracted the effects of let-7a expression. In conclusion, Lin28 promotes cell proliferation by regulating mRNA translation (let-7-independent) and miRNA biogenesis (let-7-dependent). Lin28 can tightly control HDPC proliferation by promoting the expression of pro-proliferative genes by directly enhancing their translation.
Here, it was shown that the levels of Lin28 mRNA and protein in lentivirus-Lin28 (LV-Lin28)-infected HDPCs were significantly increased compared with uninfected HDPCs, while there was little difference between the lentivirus-control (LV-CN)-infected and control groups (Figure 1A). The absorbance data of the CCK-8 assay showed that the cell proliferation of LV-Lin28-infected HDPCs was significantly increased at 48 hours compared with the control cells (Figure 1B). The cell proliferation of LV-Lin28-infected HDPCs was further enhanced at 72 hours. Using EdU detection, it was found that the percentage of EdU-positive cells in LV-Lin28-infected cells was significantly increased compared with the control cells (Figure 1C), indicating that cell viability and cell growth in HDPCs with stable overexpression of Lin28 were significantly promoted. In summary, these results indicate that Lin28 promotes the cell proliferation of HDPCs.
Figure 1. Lin28 promotes the cell proliferation of HDPCs. (Liu Y, et al., 2019)
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