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Human PLA2G2A adenoviral particles

Human PLA2G2A adenoviral particles

Cat.No. :  AD00230Z

Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL

Storage:  -80℃ Shipping:  Frozen on dry ice

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Adenovirus Particle Information

Quality Control

Gene Informationn

Cat. No. AD00230Z
Target Gene PLA2G2A
Species Human
Product Type Adenoviral particle
Insert PLA2G2A
Titer Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc.
Size Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots.
Endotoxin Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance.
Sterility Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination.
Ad5 E1 Detection All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination.
RCA Assays Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources.
PFU Titering All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells.
Gene Name
Gene Symbol
Synonyms
Gene ID
UniProt ID
mRNA Refseq
Chromosome Location
Function
Pathway
MIM
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The PLA2G2A gene encodes group IIA secretory phospholipase A2 (sPLA2-IIA), a calcium-dependent enzyme that hydrolyzes sn-2 phospholipids to release free fatty acids and lysophospholipids. The protein plays a key role in inflammatory responses, lipid metabolism, and host defense mechanisms. Elevated expression of PLA2G2A has been associated with a variety of pathological conditions, including atherosclerosis, rheumatoid arthritis, and cancer, and it is involved in regulating cell proliferation, apoptosis, and immune regulation. Its enzymatic activity and signaling properties make it a potential target for drug development, especially in the field of inflammation-related diseases. Human PLA2G2A adenoviral particles are recombinant adenoviral vectors designed to deliver the PLA2G2A gene to target cells for overexpression studies. These particles take advantage of the high transduction efficiency and broad tropism of adenoviruses to enable stable gene expression in both dividing and non-dividing cells. The adenoviral backbone is often modified to ensure safety, such as deletion of the E1/E3 regions, rendering the virus replication-incapable while maintaining high levels of transgene expression. PLA2G2A adenoviral particles are widely used in in vitro and in vivo studies to investigate the role of this gene in inflammation, cancer progression, and lipid-mediated signaling pathways. Applications include functional analysis, mechanistic studies, and preclinical evaluation of the therapeutic potential of PLA2G2A.
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Customer Reviews
Highly Efficient Transfection

The adenoviral particles delivered excellent transfection efficiency in our cell lines. The PLA2G2A expression was robust and consistent—perfect for our research on inflammatory pathways!

Canada

09/16/2021

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