Cat.No. : AD00229Z
Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | AD00229Z |
| Target Gene | PINK1 |
| Species | Human |
| Product Type | Adenoviral particle |
| Insert | PINK1 |
| Titer | Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc. |
| Size | Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance. |
| Sterility | Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination. |
| Ad5 E1 Detection | All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination. |
| RCA Assays | Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources. |
| PFU Titering | All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells. |
| Gene Name | PINK1 PTEN induced putative kinase 1 [ Homo sapiens ] |
| Gene Symbol | PINK1 |
| Synonyms | BRPK; PARK6 |
| Gene Description | PTEN induced putative kinase 1 |
| Gene ID | 65018 |
| UniProt ID | Q9BXM7 |
| mRNA Refseq | NM_032409.2 |
| Protein Refseq | NP_115785.1 |
| Chromosome Location | 1p36 |
| Pathway | Parkinsons disease, organism-specific biosystem; |
| MIM | 608309 |
Acute-on-chronic liver failure (ACLF) is a fatal syndrome with high short-term mortality. Increasing evidence suggests that apoptosis plays a crucial role in the progression of liver failure. PINK1 plays a crucial role in maintaining cell survival. However, the role and underlying mechanism of PINK1 in apoptosis in ACLF are not fully understood. Here, researchers found that PINK1 significantly improved ACLF, characterized by decreased aspartate aminotransferase (AST) and alanine aminotransferase (ALT), and improved gross and microscopic histopathological appearance of liver tissue. Meanwhile, PINK1 affected the expression of cleaved caspase-3 through mTORC2/AKT, and this effect was abolished after further intervention with Rictor or AKT. Overall, these findings suggest that PINK1 is involved in regulating multiple biological functions, including hepatocyte growth and apoptosis in ACLF, through the mTORC2/AKT signaling pathway.
First, the researchers examined the effects of PINK1 overexpression and knockdown on cell survival, respectively. L02 cells were transfected with adenovirus overexpressing PINK1 (Ad-PINK1), PINK1-specific siRNA (shPINK1), and a no-load control virus. CCK8 assays were performed to examine the effects of Ad-PINK1 and shPINK1 on H2O2-induced cell proliferation. CCK8 assays showed that PINK1 overexpression promoted the proliferation of L02 cells after H2O2 injury. In contrast, PINK1 knockdown significantly inhibited cell proliferation (Figure 1A). To explore the downstream targets of PINK1, the researchers examined changes in AKT phosphorylation levels. As shown in Figure 1B, PINK1 overexpression significantly enhanced the phosphorylation of Akt at Ser-473. This result was confirmed by immunostaining to detect phosphorylated Akt.
Figure 1. PINK1 regulates cell proliferation and enhances the phosphorylation level of Akt. (Yin X, et al., 2022)
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