Cat.No. : AD00182Z
Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | AD00182Z |
| Target Gene | HDAC1 |
| Species | Human |
| Product Type | Adenoviral particle |
| Insert | HDAC1 |
| Titer | Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc. |
| Size | Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance. |
| Sterility | Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination. |
| Ad5 E1 Detection | All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination. |
| RCA Assays | Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources. |
| PFU Titering | All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells. |
Overproduction of visceral fat is a major risk factor for many diseases. Inhibiting adipogenesis of mesenchymal stem cells (MSCs) would be an effective approach to prevent adipogenesis. Here, researchers elucidated that POU class 2 homeobox-related factor 1 (POU2AF1) may promote adipogenesis of MSCs through histone deacetylase 1 (HDAC1) signaling. Human retroperitoneal adipose-derived MSCs were isolated from overweight patients and controls. Overexpression and interference of POU2AF1 or HDAC1 were achieved using adenoviral transduction and cell small interfering RNA transfection. POU2AF1 expression was upregulated in the retroperitoneal adipose tissue of overweight patients and increased during adipogenesis. Overexpression of POU2AF1 promoted spontaneous adipogenesis without adipogenic treatment. Silencing of endogenous POU2AF1 in MSCs inhibited adipogenesis. POU2AF1 overexpression alleviated the translocation of HDAC1 to the nucleus, and the mRNA level of HDAC1 was also reduced. Co-transfection of Ad-POU2AF1 and Ad-HDAC1 partially reversed the promoting effect of POU2AF1 overexpression on spontaneous adipogenic differentiation of MSCs. POU2AF1 is involved in the natural differentiation of human mesenchymal stem cells, and overexpression or silencing of POU2AF1 can effectively induce or inhibit HDAC1 signal-mediated adipogenesis.
To determine whether HDAC1 is the main mediator of POU2AF1-regulated adipogenic differentiation, the researchers co-transfected Ad-POU2AF1 and Ad-HDAC1 and compared them with Ad-HDAC1 alone. Overexpression of HDAC1 was verified by Western blotting and real-time PCR analysis (Figure 1a-c). Co-transfection of Ad-POU2AF1 and Ad-HDAC1 partially reversed the promoting effect of POU2AF1 overexpression on spontaneous adipogenic differentiation of hASMCs (Figure 1d-g). The results showed that co-transfection of Ad-POU2AF1 and Ad-HDAC1 also reversed the protein and mRNA levels of PPARγ and C/EBPα, especially C/EBPα. These data indicate that HDAC1 is a downstream effector of POU2AF1-regulated adipogenic differentiation, while overexpression of HDAC1 alone has no such effect.
Figure 1. Overexpression of HDAC1 abolished the promotion effect of POU2AF1 on spontaneous adipogenic differentiation. (Wang Y, et al., 2021)
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The Human HDAC1 adenoviral particles from Creative Biogene exceeded my expectations. The packaging was secure, and the product arrived on time with clear documentation.
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