Human DRD2-SNAP Stable Cell Line-CHO

Name: Human DRD2-SNAP Stable Cell Line-CHO
SKU: CSC-RG0047
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0047

Host Cell : CHO Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0047
Background	This gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing.
Target Gene	DRD2
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuDRD2-SNAP
Alias	D2R, D2DR, DRD2
Host Cell	CHO
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	DRD2 dopamine receptor D2 [ Homo sapiens ]
Gene Symbol	DRD2
Synonyms	DRD2; dopamine receptor D2; D dopamine receptor; dopamine D2 receptor; dopamine receptor D2 isoform; seven transmembrane helix receptor; D2R; D2DR;
GeneID	1813
Uni ProtID	P14416
mRNA Refseq	BC021195
Chromosome Location	11q22-q23
Function	G-protein coupled receptor activity; dopamine binding; dopamine receptor activity, coupled via Gi/Go; dopamine receptor activity, coupled via Gi/Go; drug binding; drug binding; ionotropic glutamate receptor binding; potassium channel regulator activity; protein binding; receptor activity; receptor binding; signal transducer activity;
Pathway	Amine ligand-binding receptors, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Cocaine addiction, organism-specific biosystem; Cocaine addiction, conserved biosystem; Dopamine receptors, organism-specific biosystem; Dopaminergic synapse, organism-specific biosystem; Dopaminergic synapse, conserved biosystem;
MIM	126450

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Customer Q&As

What are the future directions for research using the Human DRD2-SNAP Stable Cell Line-CHO, and what new insights can we expect to gain?

A: Future research with the Human DRD2-SNAP Stable Cell Line-CHO may involve the development of advanced imaging techniques, the integration of CRISPR/Cas9 for precise genetic editing, and the use of high-throughput screening methods. These advancements could lead to a deeper understanding of DRD2's role in brain function and disease, the discovery of novel therapeutic targets, and the optimization of existing treatments.

How does the Human DRD2-SNAP Stable Cell Line-CHO contribute to the development of personalized medicine in the context of DRD2-related diseases?

A: The Human DRD2-SNAP Stable Cell Line-CHO can be used to study individual patient-derived cell lines, allowing for the investigation of how genetic variations in DRD2 affect drug responses. This personalized approach can lead to the development of tailored treatments for patients with DRD2-related conditions, improving therapeutic outcomes and reducing adverse effects.

How does the Human DRD2-SNAP Stable Cell Line-CHO aid in the study of DRD2 receptor signaling pathways?

A: The Human DRD2-SNAP Stable Cell Line-CHO enables researchers to study the downstream signaling cascades activated by DRD2 in response to dopamine or other ligands. By using this cell line, scientists can investigate the intracellular signaling networks, protein-protein interactions, and cellular responses that are modulated by DRD2 activation.

What are the technical challenges in maintaining the stability of the Human DRD2-SNAP Stable Cell Line-CHO, and how are they addressed?

A: Maintaining the stability of the DRD2-SNAP fusion protein over time can be challenging due to potential genetic drift or selection pressure. Researchers address this by using appropriate selection markers, regular monitoring of the fusion protein expression, and periodic recloning to ensure the cell line retains its original characteristics.

Can the Human DRD2-SNAP Stable Cell Line-CHO be used to study the effects of drugs on DRD2 receptors, and how does this inform drug development?

A: Yes, the Human DRD2-SNAP Stable Cell Line-CHO is a powerful tool for drug screening and pharmacological studies. By observing how drugs interact with the DRD2 receptor, researchers can evaluate their efficacy, selectivity, and potential side effects. This information is critical for the development of new therapeutics targeting DRD2-related disorders.

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