Human CD160 Stable Cell Line-CHO-K1

Name: Human CD160 Stable Cell Line-CHO-K1
SKU: CSC-RO0042

Cat.No. : CSC-RO0042 Host Cell: CHO-K1

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0042
Description	This cell line is a stably transfected cell line which expresses Human CD160 without any tag.
Gene	CD160
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Stability	Validated for at least 10 passages
Application	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Shipping	Dry ice
Storage	Liquid nitrogen

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

Publications

Q & A

Customer Reviews

CD160 is an 27 kDa glycoprotein which was initially identified with the monoclonal antibody BY55. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. The cDNA sequence of CD160 predicts a cysteine-rich, glycosylphosphatidylinositol-anchored protein of 181 amino acids with a single Ig-like domain weakly homologous to KIR2DL4 molecule. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. RNA blot analysis revealed CD160 mRNAs of 1.5 and 1.6 kb whose expression was highly restricted to circulating NK and T cells, spleen and small intestine. Within NK cells CD160 is expressed by CD56dimCD16+ cells whereas among circulating T cells its expression is mainly restricted to TCRgd bearing cells and to TCRab+CD8brightCD95+CD56+CD28-CD27-cells. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and nonclassical MHC class I molecules.

Customer Q&As

What is the genetic modification strategy used to create the Human CD160 Stable Cell Line-CHO, and how does it ensure stable expression of the CD160 protein?

A: The Human CD160 Stable Cell Line-CHO is generated through genetic engineering techniques that involve the stable integration of the CD160 gene into the CHO cell genome. This is typically achieved using vectors containing selectable markers and regulatory elements that drive the expression of the CD160 gene. The strategy ensures stable expression by incorporating the gene into the host cell's chromosomes, which allows for consistent production of the CD160 protein across multiple cell divisions.

How does the Human CD160 Stable Cell Line-CHO contribute to the study of immune cell interactions, particularly in the context of natural killer (NK) cell function?

A: The Human CD160 Stable Cell Line-CHO is instrumental in studying immune cell interactions as CD160 is a receptor expressed on NK cells that plays a role in their activation and function. By providing a cell line with stable CD160 expression, researchers can investigate the role of this receptor in NK cell recognition, signaling, and cytotoxicity, leading to a better understanding of immune responses.

What are the key considerations for maintaining the genetic stability and phenotypic characteristics of the Human CD160 Stable Cell Line-CHO during long-term culture?

A: To maintain the genetic stability and phenotypic characteristics of the Human CD160 Stable Cell Line-CHO, it is crucial to monitor the cell line regularly for potential genetic drift or changes in CD160 expression levels. This includes periodic testing for mycoplasma contamination, verifying the expression of CD160, and ensuring optimal culture conditions that prevent stress and promote cell health.

How does the Human CD160 Stable Cell Line-CHO aid in the development of therapeutics targeting CD160, and what are the potential challenges in this process?

A: The Human CD160 Stable Cell Line-CHO is valuable for the development of therapeutics as it provides a platform for high-throughput screening of compounds that modulate CD160 activity. Challenges include ensuring the stability of the cell line, the specificity of the compounds, and the translation of in vitro results to in vivo efficacy and safety.

What are the ethical considerations when using the Human CD160 Stable Cell Line-CHO in research, and how do these align with regulatory guidelines?

A: The use of the Human CD160 Stable Cell Line-CHO in research must adhere to ethical guidelines that protect human dignity and welfare. This includes obtaining informed consent for the use of human-derived materials, ensuring that the cell line is used for research purposes only, and following guidelines set by institutional review boards (IRBs) and relevant regulatory bodies.

What are the challenges associated with maintaining the Human CD160 Stable Cell Line-CHO in long-term culture, and how can these be mitigated?

A: Long-term culture of the Human CD160 Stable Cell Line-CHO may be associated with challenges such as genetic drift, loss of protein expression, and potential contamination. Regular monitoring and quality control measures, including testing for mycoplasma contamination and verifying CD160 expression, are essential to maintain the cell line's integrity.

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