Cat.No. : CSC-RO0124 Host Cell: Ba/F3
Size: >1x10^6 frozen cells/vial, 1 mL Stability: Stable in culture over a minimum of 10 passages
| Cat. No. | CSC-RO0124 |
| Description | Ba/F3-BCR-ABL cell line is a stably transfected cell line which expresses human BCR-ABL fusion protein. |
| Target Gene | BCR-ABL |
| Gene Species | Homo sapiens (Human) |
| Host Cell | Ba/F3 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Growth Conditions | 37 °C, 5% CO2 |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Size | >1x10^6 frozen cells/vial, 1 mL |
| Biosafety Level | 2 |
| Thawing & Subculturing Instructions | 1. Thaw cells by gently swirling in a 37°C water bath. To limit contamination, do not submerge the O-ring and cap. 2. When cells are ~70% thawed (~1 min), transfer the vial into a biosafety cabinet, and wipe the surface with 70% ethanol. Allow tube to dry completely. 3. Transfer the cells gently into a 15 mL conical tube containing 10 mL of pre-warmed culture medium (without antibiotic selection marker). Centrifuge cells at ~125 x g for 5~7 min. 4. Remove supernatant without disturbing the pellet, and resuspend cells in 1 mL culture medium (without antibiotic selection marker). Transfer cells to a 6-well plate containing ~2 mL pre-warmed growth medium (without antibiotic selection marker) or a T25 flask containing 5 mL pre-warmed culture medium (without antibiotic selection marker). 5. Incubate the culture at 37°C with 5% CO2. 6. Subculture: split saturated culture 1:4 ~ 1:6 every 3 days; seed out at about 1~3 x 10^5 cells/mL. |
| Growth Properties | Suspension, round |
| Freeze Medium | Frozen with 70% medium, 20% FBS, 10% DMSO |
| Freezing Instructions | Cells are recommended to generate additional frozen stocks at early passages. Frozen stocks should be preserved in a designated cryopreservation medium or in 70% RPMI 1640 + 20% FBS + 10% DMSO (without antibiotic selection marker). 1. Prepare the freezing medium (70% RPMI 1640 + 20% FBS + 10% DMSO, without antibiotic selection marker) fresh immediately before use. 2. Keep the freezing medium on ice and label cryovials. 3. Transfer cells to a sterile, conical centrifuge tube, and count the cells. 4. Centrifuge the cells at 250 x g for 5 minutes at room temperature and carefully aspirate off the medium. 5. Resuspend the cells at a density of at least 3 x10^6 cells/ml in chilled freezing medium. 6. Aliquot 1 ml of the cell suspension into each cryovial. 7. Freeze cells in the CoolCell freezing container overnight in a -80°C freezer. 8. Transfer vials to liquid nitrogen for long-term storage. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Chronic myeloid leukemia (CML) is characterized by the presence of the Bcr-Abl fusion gene, a hallmark of this subtype of myeloid leukemia. With the aid of immunoprecipitation binding mass spectrometry, the researchers were able to identify important proteins interacting with the Bcr-Abl CC domain. They used Deep Viewer to anticipate the precise binding location, and an immunoprecipitation experiment was used to confirm it. The subcellular localization was observed using western blot and IF. The Bcr-Abl CC domain was observed to bind with HSP90AB1, which prevented its nuclear transport and preserved Bcr-Abl tyrosine kinase activation. As a result, CML cells had reduced proliferation and were driven to undergo apoptosis. Furthermore, there was a considerable reduction in CML cell proliferation when 17AAG and Leptomycin B were combined.
Figure 1. The researchers utilized BCR-ABL Stable Cell Line construction to investigate cellular mechanisms. Using Lipofectamine2000, plasmids encoding HSP90AB1 and BCR-ABL variations were transfected into cells. Western blot analysis and DNA sequencing were used for validation. (Peng Y, et al., 2021)
A: Our human BCR-ABL stable cell line has undergone rigorous purification and validation procedures to ensure its purity and stability. We use a variety of techniques to identify cell lines, including PCR, immunofluorescence staining, and Western blot. We also regularly perform cytological testing of cell lines to ensure they are free of hybridization or mutation.
A: Our human BCR-ABL stable cell line has stable expression levels and has been widely used in research on the mechanisms of leukemia. We provide detailed experimental data support, including cell proliferation experiments, cytokine release analysis, etc. Customers can refer to these data to evaluate the performance of our products.
A: Our human BCR-ABL stable cell line has been widely used in anti-cancer drug screening experiments. We provide relevant literature support demonstrating the potential application of our products in anticancer drug screening.
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The supplier's technical support was very professional. They not only provided detailed operation guides, but also responded quickly when I encountered problems, ensuring the smooth progress of the experiment.
Human BCR-ABL Stable Cell Line - Ba/F3 is an excellent cell model particularly suitable for studying BCR-ABL-mediated leukemogenesis and treatment resistance. After expressing the BCR-ABL fusion protein, the cell line showed sustained tyrosine kinase activity, mimicking the pathological characteristics of CML patients. The rapid growth and efficient transfection characteristics of Ba/F3 cells make my research more efficient. The service of the supply platform is also very timely and professional. From the quality of the cells to the after-sales technical support, everything is very satisfactory.
The expression of BCR-ABL fusion protein enables Ba/F3 cells to grow in the absence of IL-3, which provides a stable and reliable model for my research. It is particularly worth mentioning that the supplier's service quality is very high. They provided detailed experimental support and quick response, which greatly improved my experimental efficiency.
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