Cat.No. : AD00343Z
Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | AD00343Z |
| Description | Human Adenovirus Type5 (dE1/E3) expressing Histone Deacetylase 11 with C-terminus V5 epitope tag under CMV promoter. C-terminus V5 epitope tag, pre-made adenovirus, ready to ship and ready to use format. |
| Target Gene | HDAC11 |
| Product Type | Adenoviral particle |
| Insert | HDAC11, C-fusion with V5 tag |
| Titer | Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc. |
| Size | Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance. |
| Sterility | Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination. |
| Ad5 E1 Detection | All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination. |
| RCA Assays | Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources. |
| PFU Titering | All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells. |
Inhibition of histone deacetylase 11 (HDAC11) promotes IL-10 expression in mouse macrophages RAW264.7 and induces immune tolerance. Here, researchers investigated the role of HDAC11 in the induction of immune tolerance in Kupffer cells (KCs) after orthotopic liver transplantation (OLT) in rats. KCs isolated from BALB/c mice were divided into pHDAC11, adHDAC11, and pCV groups (treated with HADC11-shRNA, adenovirus encoding HDAC11, and control vector, respectively). After Ad-HDAC11 and pHDAC11 treatment, rats were subjected to OLT. In KCs, Ad-HDAC11 and HDAC11-shRNA inhibited and promoted IL-10 expression, respectively. Compared with the pCV group, MHC-II class molecules and co-stimulatory molecules on the surface of KCs and T cell proliferation were significantly inhibited and induced in the pHDAC11 and Ad-HDAC11 groups. Compared with the pCV group, the serum levels of IL-2, TNF-α, and IFN-γ in the pHDAC11 group were significantly decreased, while the serum levels of IL-4 and IL-10 in the Ad-HDAC11 group were significantly increased. These results suggest that inhibition of HDAC11 can promote the expression of IL-10 in KCs after rat liver transplantation and induce hepatocyte tolerance. Therefore, HDAC11 may be a key component of this immune regulatory system and a promising target for the development of new gene therapy drugs to induce tolerance in clinical liver transplantation.
Recipients were sacrificed to evaluate histologic changes at indicated time points. Light microscopy showed that the transplanted kidneys of rats in the Ad-HDAC11 group underwent acute rejection, while the rejection in the pHDAC11 group was mild (Figure 1). ALT (U/L) and TBIL (mmol/L) of rats in the Ad-HDAC11 group increased significantly, and ALB (g/L) decreased, indicating severe liver damage. However, the above liver function indicators of rats in the pHDAC11 group changed slightly, indicating that the liver damage of rats in the pHDAC11 pretreatment group was mild.
Figure 1. Pathologic alterations of each group at 3 d after OLT, light microscopy (LM) hematoxylin-eosin (HE) X400. (Lian Z, et al., 2012)
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The HDAC11 adenovirus from Creative Biogene delivered outstanding transduction efficiency in our neuronal studies. The purity and titer were perfect, and the results were highly reproducible. Highly recommend for epigenetic research!
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