Cat.No. : CSC-RT0103
Host Cell: CHO-K1 Target Gene: GlulBaxBak1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0103 |
| Description | CHO-K1-GS/Bax/Bak cell line is a stable cell line with a homozygous knockout of GS, Bax and Bak1 |
| Target Gene | GlulBaxBak1 |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
I am thoroughly impressed with the performance of GS/Bax/Bak1 Knockout Cell Line-CHO-K1. Its exceptional knockout efficiency has allowed me to generate reliable knockout models and delve deeper into the functional role of specific genes.
Using GS/Bax/Bak1 Knockout Cell Line-CHO-K1 has been a with outstanding competence helper in my research. Its reliable and reproducible knockout results have provided me with a solid foundation for studying gene function and unraveling complex biological pathways.
GS/Bax/Bak1 Knockout Cell Line-CHO-K1 has simplified the gene editing process and saved me valuable time and resources. Its user-friendly interface and high knockout efficiency have made gene manipulation experiments more efficient and successful.
I cannot emphasize enough how invaluable GS/Bax/Bak1 Knockout Cell Line-CHO-K1 has been in my studies. Its consistent and efficient knockout capabilities have opened up new avenues of investigation and propelled my research forward.
I wholeheartedly recommend GS/Bax/Bak1 Knockout Cell Line-CHO-K1 to researchers seeking a reliable and effective tool for gene editing. Its outstanding performance, ease of use, and consistent knockout outcomes have greatly enhanced the quality and impact of my research.
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