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Cat.No. : CSC-RR0125 Host Cell: HepG2
| Cat. No. | CSC-RR0125 |
| Description | Hep G2 is a perpetual cell line which was derived from the liver tissue of a 15-year-old Caucasian American male with a well-differentiated hepatocellular carcinoma. These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice. Hep G2 cells express 3-hydroxy-3-methylglutaryl-CoA reductase and hepatic triglyceride lipase activities. Hep G2 cells demonstrate decreased expression of apoA-I mRNA and increased expression of catalase mRNA in response to gramoxone (oxidative stress). There is no evidence of a Hepatitis B virus genome in this cell line. The GFP Stable Cell Line-HepG2 constitutively expresses GFP. |
| Host Cell | HepG2 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Recommended Medium | Inquiry for instruction of culturing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
TMEM2 expression is down-regulated in liver tissues of patients with chronic hepatitis B virus (HBV) infection and HepG2.2.15 cells carrying HBV genomic DNA. In the present study, the researchers further explored the effects of TMEM2 on HepG2 and HepG2.2.15 cells during HBV infection and its mechanism. By using lentiviral vectors, the researchers established HepG2 shTMEM2 cells with stable knockdown of TMEM2 as well as HepG2 TMEM2 and HepG2.2.15 TMEM2 cells with stable overexpression of TMEM2. The results showed that TMEM2 expression was reduced in HBV-infected liver tissues and HepG2.2.15 cells. In TMEM2 knockdown HepG2 shTMEM2 cells, HBsAg, HBcAg, HBV DNA, and HBV cccDNA levels were significantly increased, whereas these levels were significantly decreased in HepG2 TMEM2 and HepG2.2.15 TMEM2 cells overexpressing TMEM2. The findings suggest that TMEM2 inhibits HBV infection in HepG2 and HepG2.2.15 cells through activation of the JAK-STAT signaling pathway.
Figure 1. Effects of TMEM2 overexpression or silencing on HBsAg, HBcAg, HBV DNA, and HBV cccDNA levels after HBV infection in HepG2 cell lines with stable GFP expression. (Zhu X, et al., 2016)
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I used the GFP Stable Cell Line HepG2 product in the experiment. Due to its unique labeling characteristics, this product enables the expressed cell lines to be efficiently expressed not only in liver cell research, but also in multiple clinical fields, providing a broader exploration space.
United Kingdom
04/03/2020
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