Eco52I

Name: Eco52I
SKU: EROT0338

For research use only. Not intended for any clinical use.

Cat.No.

EROT0338

Reaction Buffer

10mM Tris-HCl (pH 8.5 at 37 °C), 3mM MgCl2, 100mM NaCl and 0.1mg/mL BSA

Reaction Conditions

Incubate at 37 °C

Recognition Site

C↓GGCCG

Size/Form

500 Units;1000 Units

Storage

10mM Tris-HCl (pH7.4 at 25 °C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Publications

Q & A

Customer Reviews

Customer Q&As

Is Eco52I suitable for high-throughput genome editing experiments, and what are its advantages and disadvantages in this regard?

A: Eco52I may be suitable for high-throughput genome editing experiments. Its advantages may include high cutting efficiency and specificity, while disadvantages may include the need for optimized reaction conditions and possible cellular toxicity.

Can the cutting selectivity of Eco52I toward DNA be improved through specific modification methods?

A: Yes, through specific modification methods, such as using modified nucleotides or adding specific cofactors, the cutting selectivity of Eco52I toward DNA may be affected.

Is there a significant difference in the cutting efficiency of Eco52I when dealing with supercoiled DNA and linear DNA?

A: The cutting efficiency of Eco52I may vary when dealing with supercoiled DNA and linear DNA, and the specific cutting efficiency needs to be determined through experiments.

Does the enzymatic activity of Eco52I significantly decrease after long-term storage? How to evaluate?

A: The enzymatic activity of Eco52I may decrease after long-term storage. Its enzymatic activity can be evaluated through cutting experiments compared with fresh enzyme, as well as utilizing enzymatic activity assays.

How is the cutting specificity of Eco52I affected by reaction conditions, such as reaction temperature and salt concentration?

A: The cutting specificity of Eco52I may be affected by reaction conditions. Different reaction temperatures and salt concentrations may affect the binding affinity and cutting efficiency of the enzyme with DNA, thereby affecting its cutting specificity.

How to optimize the cutting efficiency of Eco52I during DNA cleavage experiments?

A: Optimizing the cutting efficiency of Eco52I can be achieved by adjusting reaction conditions such as reaction temperature, buffer system, and enzyme concentration, as well as ensuring the purity and integrity of the DNA template.

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