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XhoII

For research use only. Not intended for any clinical use.
Cat.No.
EROT0493
Description
Xanthomonas holcicola.
Concentration
5–10u/μl
Reaction Conditions
Buffer C. 37 °C.
Recognition Site
R↓GATCY
Size/Form
100 units;500 units
Storage
10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

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Q & A

Customer Reviews

Customer Q&As
What are the cleavage sites of the enzyme?

A: The restriction endonuclease recognizes the enzyme cutting site 5'-C↑TCGAG-3' and has optimal activity in the W buffer solution.

What is the typical length of time for enzyme digestion?

A: If the enzyme is HIFI, it can be digested for 2 hours at 37℃, if it is a regular enzyme, it needs to be digested for 3-4 hours, if there is a problem with the experimental schedule, it can also be digested overnight at 37℃.

From which strain is this enzyme extracted?

A: The enzyme is obtained from recThe enzyme is obtained from recombinant E.coli carrying xhoIR gene from Streptococcus.

Will there be a frameshift issue when using the enzyme?

A: Typically, there is no frameshift mutation, but when designing primers, it's common to add three nucleotides as protection.

Will methylation affect the enzymatic cleavage efficiency during enzyme digestion?

A: In some cases, it may. The enzyme is dam methylation-insensitive, dcm methylation-insensitive, but CpG methylation-sensitive.

Under what conditions will the enzyme be inactivated?

A: The enzyme will lose activity when heated at 80°C for 20 minutes.

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Customer Reviews
Easy to use, can be combined in any way

Suitable for universal Single Buffer buffer, which can be arbitrarily combined within a unified reaction system, without multiple single digestion or changing digestion buffer, convenient, stable and fast, and can cut vector precisely.

United States

01/22/2023

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