Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EROT0493
| Cat. No. | EROT0493 |
| Description | Xanthomonas holcicola. |
| Concentration | 5–10u/μl |
| Recognition Site | R↓GATCY |
| Size | 100 units;500 units |
| Reaction Conditions | Buffer C. 37 °C. |
| Storage | 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol. |
A: The restriction endonuclease recognizes the enzyme cutting site 5'-C↑TCGAG-3' and has optimal activity in the W buffer solution.
A: If the enzyme is HIFI, it can be digested for 2 hours at 37℃, if it is a regular enzyme, it needs to be digested for 3-4 hours, if there is a problem with the experimental schedule, it can also be digested overnight at 37℃.
A: The enzyme is obtained from recThe enzyme is obtained from recombinant E.coli carrying xhoIR gene from Streptococcus.
A: Typically, there is no frameshift mutation, but when designing primers, it's common to add three nucleotides as protection.
A: In some cases, it may. The enzyme is dam methylation-insensitive, dcm methylation-insensitive, but CpG methylation-sensitive.
A: The enzyme will lose activity when heated at 80°C for 20 minutes.
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Suitable for universal Single Buffer buffer, which can be arbitrarily combined within a unified reaction system, without multiple single digestion or changing digestion buffer, convenient, stable and fast, and can cut vector precisely.
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