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CviJ I

Cat.No.
EROT0321
Recognition Site
PuG↓Cpy
Source Description
Chlorella virus IL-3A
Unit Definition
One unit is the amount of enzyme required to completely digest 1 μg of pBR322 DNA in 1 hour in a total reaction volume of 25 μl.
Reaction Conditions
Incubation at 37 °C
Reaction Buffer
20 mM glycylglycine-KOH (pH 8.5)10 mM magnesium acetate50 mM potassium acetate0.1 mM dithiothreitolNote 2: 100% DMSO supplied separately
Size
400 Units;100 Units
Storage Buffer
20 mM Tris-acetate (pH 8.0 at 4 °C)50 mM potassium acetate5 mM MgCl20.5 mM EDTA1 mM dithiothreitol50% (v/v) glycerol
Storage Conditions
Store at -20 °C.

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