CviJ I

Name: CviJ I
SKU: EROT0321

For research use only. Not intended for any clinical use.

Cat.No.

EROT0321

Reaction Buffer

20 mM glycylglycine-KOH (pH 8.5)10 mM magnesium acetate50 mM potassium acetate0.1 mM dithiothreitolNote 2: 100% DMSO supplied separately

Reaction Conditions

Incubation at 37 °C

Recognition Site

PuG↓Cpy

Size/Form

400 Units;100 Units

Source

Chlorella virus IL-3A

Storage

20 mM Tris-acetate (pH 8.0 at 4 °C)50 mM potassium acetate5 mM MgCl20.5 mM EDTA1 mM dithiothreitol50% (v/v) glycerol

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of pBR322 DNA in 1 hour in a total reaction volume of 25 μl.

Publications

Q & A

Customer Reviews

Customer Q&As

Under what conditions should CviJI be stored?

A: CviJI is stored in a buffer containing 20 mM potassium glycyl-glycine (pH 8.5), 10 mM magnesium acetate, 50 mM potassium acetate, and 0.1 mM dithiothreitol. It is best stored at -80°C upon receipt.

What is the reaction system for CviJI?

A: CviJI can be incubated at 37°C in a 25 μl reaction volume for 1 hour to complete the reaction.

If I need to inactivate CviJI for further reactions, what conditions or temperature should be used?

A: The optimal reaction temperature for CviJI is 37°C, and if you need to inactivate it, incubate at 65°C for 10 minutes to achieve inactivation.

Can CviJI cleave other sites?

A: CviJI cleaves only at PuGCPy sites and is suitable for high-resolution mapping of short DNA samples or small plasmids.

What applications can CviJI be used for?

A: CviJI is useful for novel research and applications such as shotgun cloning, hot-start PCR, and epitope mapping.

What are the characteristics of CviJI?

A: CviJI is the only restriction endonuclease that recognizes two three-base pair sequences.

Is CviJI the only product in this package, or are there other items?

A: In addition to CviJI, the product includes 10X CviJI Reaction Buffer and separate 100% DMSO. It is available in 400 units and 100 units packaging sizes.

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