Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Premade Virus Particles
Ready-to-Use | High Titer | Versatile Applications
Premade AAV, adenovirus, lentivirus particles, safe, stable, in stock.
Virus-Like Particles (VLPs)
Stable | Scalable | Customizable
Advanced VLPs for vaccine development (Chikungunya, Dengue, SARS-CoV-2), gene therapy (AAV1 & AAV9), and drug screening (SSTR2, CCR5).
Oligonucleotide Products
Precise | High Yield | Tailored Solutions
Accelerate your research with cost-effective LncRNA qPCR Array Technology.
RNA Interference Products
Targeted | Potent | High Specificity
Human Druggable Genome siRNA Library enables efficient drug target screening.
Recombinant Drug Target Proteins
Authentic | Versatile | Accelerated
Providing functional, high-purity recombinant proteins—including membrane proteins and nanodiscs—to overcome bottlenecks in drug screening and target validation.
Clones
Validated | Reliable | Comprehensive Collection
Ready-to-use clones for streamlined research and development.
Kits
Complete | Convenient | High Sensitivity
Chromogenic LAL Endotoxin Assay Kit ensures precise, FDA-compliant endotoxin quantification for biosafety testing.
Enzymes
Purified | Stable | Efficient
Powerful Tn5 Transposase for DNA insertion and random library construction.
Aptamers
Highly Specific | Robust | Versatile
Aptamers for key proteins like ACVR1A, Akt, EGFR, and VEGFR.
Adjuvants
Enhancing | Synergistic | Effective
Enhance immune responses with high-purity, potent CpG ODNs.
Laboratory Equipment
Innovative | Reliable | High-Precision
Effortlessly streamline DNA extraction with CB™ Magnetic-Nanoparticle Systems.
Stable Cell Line Generation
Reliable | Scalable | Customizable
Fast proposals, regular updates, and detailed reports; strict quality control, and contamination-free cells; knockout results in 4-6 weeks.
Target-based Drug Discovery Service
Innovative | Comprehensive | Efficient
Target identification, validation, and screening for drug discovery and therapeutic development.
Custom Viral Service
Versatile | High-Yield | Safe
Unbeatable pricing, fully customizable viral packaging services (covering 30,000+ human genes, 200+ mammals, 50+ protein tags).
Custom Antibody Service
Precise | Flexible | Efficient
End-to-end antibody development support, from target to validation, enabling clients to rapidly obtain application-ready antibodies.
Antibody-Drug Conjugation Service
Integrated | Controlled | Translational
Comprehensive solutions covering design, development, and validation to ensure conjugated drugs with consistent quality and clinical potential.
Protein Degrader Service
Efficient | High-Precision | Advanced Therapeutics
Harness the power of protein degraders for precise protein degradation, expanding druggable targets and enhancing therapeutic effectiveness for cutting-edge drug discovery.
Nucleotides Service
Accurate | Flexible | High-Quality
Custom synthesis of oligonucleotides, primers, and probes for gene editing, PCR, and RNA studies.
Custom RNA Service
Custom RNA ServicePrecise | Flexible | GMP-ReadyCustom
RNA design, synthesis, and manufacturing—covering mRNA, saRNA, circRNA, and RNAi. Fast turnaround, rigorous QC, and seamless transition from research to GMP production.
Custom Libraries Construction Service
Comprehensive | High-throughput | Accurate
Custom cDNA, genomic, and mutagenesis libraries for drug discovery, screening, and functional genomics.
Gene Editing Services
Precise | Efficient | Targeted
Gene editing solutions for gene editing, knockouts, knock-ins, and customized genetic modifications. Integrated multi-platform solutions for one-stop CRISPR sgRNA library synthesis and gene screening services
Microbe Genome Editing Service
Precise | Scalable | Customizable
Enhance microbial productivity with advanced genome editing using Rec-mediated recombination and CRISPR/Cas9 technologies.
Biosafety Testing Service
Reliable | Comprehensive | Regulated
Complete biosafety testing solutions for gene therapy, viral vectors, and biologics development.
Plant Genetic Modification Service
Advanced | Sustainable | Tailored
Genetic modification for crop improvement, biotechnology, and plant-based research solutions.
Plant-based Protein Production Service
Efficient | Scalable | Customizable
Plant-based protein expression systems for biopharmaceuticals, enzyme production, and research.
Aptamers Service
Innovative | Fast | Cost-Effective
Revolutionizing drug delivery and diagnostic development with next-generation high-throughput aptamer selection and synthesis technologies.
CGT Biosafety Testing
Comprehensive | Accurate | Regulatory-compliant
Internationally certified evaluation system for biologics, gene therapies, nucleic acid drugs, and vaccines.
Pandemic Detection Solutions
Rapid | Precise | Scalable
Balancing accuracy, accessibility, affordability, and rapid detection to safeguard public health and strengthen global response to infectious diseases.
cGMP Cell Line Development
Reliable | Scalable | Industry-leading
Stable expression over 15 generations with rapid cell line development in just 3 months.
Supports adherent and suspension cell lines, offering MCB, WCB, and PCB establishment.
GMP mRNA Production
Efficient | Scalable | Precise
Scalable mRNA production from milligrams to grams, with personalized process design for sequence optimization, cap selection, and nucleotide modifications, all in one service.
GMP Plasmid Production
High Quality | Scalable | Regulatory-compliant
Our plasmid production services span Non-GMP, GMP-Like, and GMP-Grade levels, with specialized options for linearized plasmids.
GMP Viral Vector Manufacturing
Scalable | High Yield | Quality-driven
Advanced platforms for AAV, adenovirus, lentivirus, and retrovirus production, with strict adherence to GMP guidelines and robust quality control.
AI-Driven Gene Editing and Therapy
Innovative | Precision | Transformative
AI-powered one-click design for customized CRISPR gene editing strategy development.
AI-Antibody Engineering Fusion
Next-Generation | Targeted | Efficient
AI and ML algorithms accelerate antibody screening and predict new structures, unlocking unprecedented possibilities in antibody engineering.
AI-Driven Enzyme Engineering
Smart | Efficient | Tailored
High-throughput enzyme activity testing with proprietary datasets and deep learning models for standardized and precise enzyme engineering design.
AI-Enhanced Small Molecule Screening
Predictive | Efficient | Insightful
Leverage AI to uncover hidden high-potential small molecules, prioritize leads intelligently, and reduce costly trial-and-error in early drug discovery.
AI-Driven Protein Degrader Drug Development
Innovative | Targeted | Accelerated
Use AI-guided design to optimize protein degraders, addressing design complexity and enhancing efficacy while shortening development timelines.
In the rapidly evolving landscape of genomic medicine, CRISPR technology is a breakthrough innovation, revolutionizing our approach to genetic engineering and therapeutic development. At Creative Biogene, we harness CRISPR systems' unprecedented precision and versatility to drive the boundaries of gene research and therapeutic applications.
Creative Biogene offers vast knowledge and experience in improving CSISPR efficiency and developing applications. Our complete gene editing services include sophisticated solutions such as sgRNA design and validation, off-target efficiency analysis, gene editing cell line production, gene editing animal model development, sgRNA library screening, and RNA editing. We are devoted to offering tailored solutions to our client's diverse demands in genome engineering and functional genomics research.
Streamlined Gene Editing Validation
Comprehensive bioinformatics analysis and meticulous sgRNA screening ensure gene editing precision. Advanced computational tools help evaluate potential off-target effects, optimize guide RNA selection, and minimize genetic modification risks and experimental errors.
Reliable Cell Line Quality Assurance
A robust quality control framework extending beyond standard validation provides deep guarantees. Rigorous testing encompasses genetic verification, protein expression analysis, and sensitive mycoplasma detection, ensuring pure, functional, and genetically stable cell lines that meet the most demanding research standards.
Customized Support for Your Experimental Needs
Expert scientists offer personalized consultation, including in-depth feasibility assessments, cell transfection optimization, and sophisticated Cre-LoxP strategy design. Carefully tailored approaches address unique research challenges, providing flexible and innovative solutions.
Optimized CRISPR Delivery for Maximum Efficiency
Cutting-edge strategies optimize every critical stage of the gene editing process. From advanced vector design to refined viral transduction techniques, the goal is to maximize genetic modification efficiency while maintaining the highest precision and cellular viability standards.
SgRNA Design and Validation
Advanced bioinformatics-driven service for high-quality sgRNA design. Optimizes sequence efficiency and specificity for precise CRISPR/Cas9 gene editing.
Off-Target Analysis
Comprehensive screening using advanced platforms to assess potential genomic modification risks. Provides detailed off-target risk assessment reports.
Gene-Edited Cell Line Construction
Complete service for generating precise gene-edited cell lines. Supports knockouts, knock-ins, and site-directed mutations with comprehensive validation.
Gene-Edited Animal Model Construction
Versatile gene-editing services for multiple animal models. Offers knockout, mutation, and knock-in strategies with complete model validation.
CRISPR Library Screening
End-to-end high-throughput screening service. Provides pathway and whole-genome libraries with comprehensive workflow from library creation to candidate validation.
RNA Editing
Precise RNA editing solutions using CRISPR-Cas13 technology. Supports base editing and RNA modification analysis with full technical support.
At Creative Biogene, we prioritize quality, precision, and customer satisfaction, delivering tailored products and services that help accelerate the translation of research into industry applications. Our clients' breakthroughs across various fields are frequently published in global journals.
Excessive fibrosis is a key factor in many organ dysfunctions, with fibroblasts differentiating into myofibroblasts, leading to increased α-SMA expression and collagen production. This process, while initially part of tissue repair, can become pathological in fibrotic diseases. Targeting the differentiation of fibroblasts into myofibroblasts is critical to mitigating fibrosis. Researchers sought to explore the transcription factor AP-2α (TFAP2A) as a potential target. Creative Biogene assisted in developing a TFAP2A knockout cell line using CRISPR/CAS9 technology. The TFAP2A-KO model enables researchers to investigate the regulation of fibrosis and evaluate therapeutic interventions.
Figure 1. The TFAP2A gene was deleted in NIH/3T3 fibroblasts using CRISPR/Cas9-based gene editing, with validation through PCR and immunoblotting analysis. (Ross GR, et al., 2019)
An O2-sensing diguanylate cyclase broadly affects the aerobic transcriptome in the phytopathogen Pectobacterium carotovorum. P. carotovorum is a significant plant pathogen that causes bacterial soft rot in crops, with its ability to sense oxygen playing a crucial role in disease progression. A key protein involved in oxygen sensing, Pcc DgcO, regulates the production of c-di-GMP, a second messenger that influences bacterial functions. The researchers used RNA sequencing and mass spectrometry to study the effects of DgcO deletion on gene expression and metal content, providing valuable insights into oxygen-dependent regulation. The researchers utilized Creative Biogene's gene editing services to construct the PccDgcO deletion strain (ΔdgcO).
Figure 2. The researchers used heatmaps to compare transcript levels related to flagellar and metal homeostasis functions between WT and ΔdgcO strains, observing decreased flagellar regulator and metal transporter transcripts. (Fekete FJ, et al., 2023)
Congenital abnormalities of the kidney and urinary tract (CAKUT) are a type of birth abnormality that affects kidney and urinary tract development. The researchers aimed to identify and functionally interpret miRNAs most frequently impacted by rare copy number variants (CNVs) in CAKUT. They used gene editing services provided by Creative Biogene to construct HEK293 cell lines with a knockout of the MIR484 gene. This knockout model, developed using our CRISPR/Cas9-based gene editing technology, enabled the researchers to investigate how CNVs affect miRNA expression and target gene regulation.
Figure 3. The researchers compared the relative mRNA expression levels of target genes between MIR484+/− KO and HEK293 WT cells, using GAPDH as an endogenous control and performing statistical analysis with Student's t-test. (Mitrovic K, et al., 2022)
We provide comprehensive support throughout the service process, including detailed parameter information and product specifications. For every service, we ensure clear documentation, including protocol guidelines, product datasheets, and any relevant technical parameters, to help you understand the specifics of the products and services. The following service durations are provided as reference timelines:
| Service Item | Service Details | Duration |
| SgRNA Design | Design sgRNA sequences for target gene groups (number of sequences can be selected). | 1-2 weeks |
| Off-Target Analysis | Genome-wide off-target analysis, Detection, and quantification of off-target effects using in vitro assays, single-molecule, computational, and crystallographic methods | 4-6 weeks |
| Gene Knockout/in Cell Line | Single-gene knockout/in, Multi-gene knockout/in, Large fragment deletion/insertion. | 9-15 weeks |
| Gene Editing Animal Model Construction | Mouse Model, Rat Model, Zebrafish Model, Nematode Model | Not specified |
| Site-Directed Mutagenesis Cell Line | Point mutations in target gene base sequences, including all base deletions, insertions, and substitutions. | 12-25 weeks |
| SgRNA Library Construction | Clone sgRNAs into the appropriate target vector. | 2-3 weeks |
| Library Preparation & Packaging | Perform transfection and collect viral particles based on shipment quantity. | 1 week |
| Library QC & NGS Validation | Perform quality control of the library, including NGS validation of uniformity and diversity (library capacity > 100x, >80% accuracy). | 2-3 weeks |
| Virus Packaging | Pack the virus with viral titer at 108 TU/ml. | 2-3 weeks |
| Library Cell Pool Construction | Build the cell pool for library construction. | 6-9 weeks |
| High-Throughput Screening | Conduct forward screening, reverse screening, drug resistance experiments, and reporter gene detection. | 2-3 weeks |
| Targeted RNA Editing | crRNA design and synthesis, CRISPR/Cas13 vector construction, Cas13 fusion protein construction | 6-12 weeks |
| RNA Base Editing | REPAIR (A-to-I editing), RESCUE (C-to-U editing), dCas13-ADAR2 fusion protein for RNA editing | 6-12 weeks |
At Creative Biogene, we are committed to creating an integrated research service platform. From primer and gene synthesis to viral packaging, cell line development, and gene editing, we provide complete solutions. Our services range from NGS to third-generation sequencing, resulting in a complete platform for gene and gene mutation functional research. We provide comprehensive services to help scientific research and the pharmaceutical sector. For the best support, please include your project type, requirements, timeline, and any special technical considerations. Let's collaborate to move your research forward—reach out to discuss your project needs with our team of experts.
Q1: What specific services are included in your sgRNA design service, and how long does it take?
A1: Our sgRNA design service offers a complete package. We design multiple sgRNA sequences based on your target gene, optimize them using bioinformatics tools to minimize off-target effects, construct and validate the sgRNA, and verify its cutting efficiency through various assays. This process usually takes 4–6 weeks, and we can customize the plan to fit your specific requirements.
Q2: Which types of cells can you handle for gene-edited cell line construction, and what is the success rate?
A2: We handle a variety of cells, including mammalian cell lines like HEK293 and HeLa, as well as stem and primary cells. The duration and complexity differ based on cell type: regular cell lines usually take 9–15 weeks, conditional knockouts take a similar time, while gene knock-ins require 12–25 weeks. We ensure cell line purity and stability through strict single-clone screening and validation.
Q3: What are your strengths in CRISPR library screening?
A3: Our strengths lie in our comprehensive genome-wide library (over 100,000 sgRNAs), a complete screening workflow from design to analysis, and professional bioinformatics support. We also offer validation services for any identified candidate genes, with the entire screening time tailored to the experimental design.
Q4: How do you ensure service quality, and what after-sales support do you provide?
A4: Quality is guaranteed by our experienced technical team, real-time project tracking, rigorous quality control, and detailed experimental reports. We provide full technical support throughout the project and offer guidance for further optimization.
Q5: What about pricing, and what are the payment options?
A5: Pricing depends on project complexity and workload. We offer detailed quotes tailored to your needs, with flexible and installment payment options. Please contact our customer service team for more specific pricing information.
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