Cat.No. : CSC-SC000765-1 Host Cell: CHO-K1
| Cat. No. | CSC-SC000765-1 |
| Description | This cell line is engineered to stably express human apolipoprotein A1 (APOA1) in CHO-K1 cells. |
| Gene | APOA1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
To explore how apolipoprotein A1 (APOA1) boosts the resistance of cervical squamous carcinoma to platinum-based chemotherapy. Researchers investigated the role of APOA1 in platinum-based chemotherapy resistance in cervical cancer. Overexpression of APOA1 reduced clone-forming ability after carboplatin treatment compared to controls. Tandem Mass Tag analysis identified 64 differentially expressed proteins downstream of APOA1. Gene Ontology analysis revealed involvement in pathways like p38 MAPK signaling via STAT1, PI3K signaling via CD81 and C3, and platinum-based chemoresistance via TOP2A.
Figure 1. The effect of APOA1 overexpression on apoptosis of SiHa/Caski cells with and without CBP was detected using a TUNEL assay. SiHa or Caski cells with control lentivirus were designated as NC, while SiHa or Caski APOA1-overexpressing cells were labeled as OE. (He Y, et al., 2022)
Utilizing Creative Biogene's Human APOA1 Stable Cell Line - CHO-K1 could enhance similar experiments by providing a stable and consistent APOA1 expression system. This eliminates the need for lentiviral transduction and ensures uniform APOA1 levels across experiments.
A: CHO-K1 cells were likely chosen due to their robust growth characteristics, ability for stable transgene expression, and suitability for protein secretion, aligning with the requirements for studying APOA1.
A: Stability was likely confirmed through methods such as immunoblotting, ELISA, or qPCR, with continuous selection pressure applied to ensure stable maintenance of APOA1 expression.
A: Characterization may involve analysis of APOA1 secretion levels, protein structure, lipid-binding properties, and functional assays to assess its role in lipid metabolism and cardiovascular health.
A: Quality control likely included screening for mycoplasma contamination, confirmation of stable transgene integration, and assessment of phenotypic stability and consistency.
A: Comparative analysis with clinical data or animal models helps validate the relevance of APOA1 expression in lipid metabolism, atherosclerosis, and other cardiovascular diseases, guiding therapeutic research efforts.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
This Human APOA1 Stable Cell Line is a gem! Its stable expression in CHO-K1 cells has revolutionized my research on cholesterol homeostasis.
Using this cell line feels like having a secret weapon! The stable APOA1 expression has made studying lipid metabolism pathways a breeze.
Can't believe how much easier my experiments have become with this cell line! Its reliable expression in CHO-K1 cells has elevated the quality of my research on cardiovascular diseases.
So impressed with this Human APOA1 Stable Cell Line! Its consistent expression has given me peace of mind and boosted the credibility of my findings.
Huge shoutout to this cell line for simplifying my work! With stable APOA1 expression, I can delve deeper into cholesterol homeostasis without any worries.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
