Human APOA1 Stable Cell Line - CHO-K1

Name: Human APOA1 Stable Cell Line - CHO-K1
SKU: CSC-SC000765-1
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC000765-1

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC000765-1
Description	This cell line is engineered to stably express human apolipoprotein A1 (APOA1) in CHO-K1 cells.
Target Gene	APOA1
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	APOA1 apolipoprotein A-I [ Homo sapiens ]
Gene Symbol	APOA1
Gene Description	apolipoprotein A-I
GeneID	335
Uni ProtID	P02647
mRNA Refseq	NM_000039.1
Protein Refseq	NP_000030.1
Chromosome Location	11q23-q24
Function	apolipoprotein A-I receptor binding; apolipoprotein receptor binding; beta-amyloid binding; cholesterol binding; contributes_to cholesterol transporter activity; cholesterol transporter activity; enzyme binding; high-density lipoprotein particle binding; high-density lipoprotein particle receptor binding; identical protein binding; phosphatidylcholine-sterol O-acyltransferase activator activity; phospholipid binding; protein binding;
Pathway	ABC-family proteins mediated transport, organism-specific biosystem; ABCA transporters in lipid homeostasis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Amyloids, organism-specific biosystem; Chylomicron-mediated lipid transport, organism-specific biosystem; Disease, organism-specific biosystem;
MIM	107680

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Background

Case Study

Applications

Q & A

Customer Reviews

The discovery of APOA1, a pivotal protein component of high-density lipoprotein (HDL), dates back to 1975, when seminal research on human plasma lipoproteins provided insights into its role. Also recognized as apolipoprotein A-I, APOA1 plays a critical function in lipid metabolism and transport, particularly within the reverse cholesterol transport pathway. Its significance lies in facilitating the removal of surplus cholesterol from peripheral tissues and its transportation to the liver for elimination. Unveiling APOA1's presence opened avenues for further exploration into its physiological functions and potential implications in cardiovascular health and pathology.

Concurrently, the establishment of a stable cell line expressing human APOA1 represented a significant advancement in biomedical inquiry. Achieved through meticulous molecular biology methodologies, this cell line serves as a valuable resource for delving into the functional roles of APOA1 in cellular and molecular mechanisms. Derived from Chinese hamster ovary cells, CHO-K1 cells possess robust growth characteristics and are amenable to genetic manipulation, rendering them an optimal platform for stable expression of human APOA1. These cell lines have played a crucial role in advancing our understanding of APOA1 biology and exploring its potential therapeutic applications in managing cardiovascular conditions.

To explore how apolipoprotein A1 (APOA1) boosts the resistance of cervical squamous carcinoma to platinum-based chemotherapy. Researchers investigated the role of APOA1 in platinum-based chemotherapy resistance in cervical cancer. Overexpression of APOA1 reduced clone-forming ability after carboplatin treatment compared to controls. Tandem Mass Tag analysis identified 64 differentially expressed proteins downstream of APOA1. Gene Ontology analysis revealed involvement in pathways like p38 MAPK signaling via STAT1, PI3K signaling via CD81 and C3, and platinum-based chemoresistance via TOP2A.

Figure 1. The effect of APOA1 overexpression on apoptosis of SiHa/Caski cells with and without CBP was detected using a TUNEL assay. SiHa or Caski cells with control lentivirus were designated as NC, while SiHa or Caski APOA1-overexpressing cells were labeled as OE. (He Y, et al., 2022)

Utilizing Creative Biogene's Human APOA1 Stable Cell Line - CHO-K1 could enhance similar experiments by providing a stable and consistent APOA1 expression system. This eliminates the need for lentiviral transduction and ensures uniform APOA1 levels across experiments.

1. Drug Screening: Assess lipid-modifying drug efficacy using Human APOA1 Stable Cell Line - CHO-K1.

2. Atherosclerosis Research: Investigate APOA1's role in atherosclerosis development and potential therapeutic targets.

3. Lipid Metabolism: Study cholesterol efflux and HDL biogenesis by manipulating APOA1 expression in the cell line.

4. Disease Modeling: Mimic dyslipidemia conditions to aid in cardiovascular disease research.

5. Genetic Engineering: Manipulate the cell line to understand APOA1 regulation for gene therapy strategies.

Customer Q&As

What were the criteria considered in selecting CHO-K1 cells for establishing the stable APOA1 cell line?

A: CHO-K1 cells were likely chosen due to their robust growth characteristics, ability for stable transgene expression, and suitability for protein secretion, aligning with the requirements for studying APOA1.

How was the stability of APOA1 expression assessed and maintained in this CHO-K1 stable cell line?

A: Stability was likely confirmed through methods such as immunoblotting, ELISA, or qPCR, with continuous selection pressure applied to ensure stable maintenance of APOA1 expression.

Can you provide insights into the characterization of APOA1 expression in the CHO-K1 stable cell line, including its secretion profile and functional implications?

A: Characterization may involve analysis of APOA1 secretion levels, protein structure, lipid-binding properties, and functional assays to assess its role in lipid metabolism and cardiovascular health.

What quality control measures were implemented during the development of this stable cell line?

A: Quality control likely included screening for mycoplasma contamination, confirmation of stable transgene integration, and assessment of phenotypic stability and consistency.

How does the expression pattern and functional properties of APOA1 in this stable cell line relate to its physiological roles and relevance in disease states?

A: Comparative analysis with clinical data or animal models helps validate the relevance of APOA1 expression in lipid metabolism, atherosclerosis, and other cardiovascular diseases, guiding therapeutic research efforts.

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Customer Reviews

Stable APOA1 expression.

This Human APOA1 Stable Cell Line is a gem! Its stable expression in CHO-K1 cells has revolutionized my research on cholesterol homeostasis.

Canada

2020-12-02

Secret weapon

Using this cell line feels like having a secret weapon! The stable APOA1 expression has made studying lipid metabolism pathways a breeze.

Canada

2020-09-11

Elevated cardiovascular disease research.

Can't believe how much easier my experiments have become with this cell line! Its reliable expression in CHO-K1 cells has elevated the quality of my research on cardiovascular diseases.

United Kingdom

2022-01-02

Credible findings

So impressed with this Human APOA1 Stable Cell Line! Its consistent expression has given me peace of mind and boosted the credibility of my findings.

French

2024-02-08

Simplified cholesterol homeostasis study

Huge shoutout to this cell line for simplifying my work! With stable APOA1 expression, I can delve deeper into cholesterol homeostasis without any worries.

French

2022-05-23

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Human APOA1 Stable Cell Line - CHO-K1

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Case Study

Applications

Q & A

Customer Reviews

What were the criteria considered in selecting CHO-K1 cells for establishing the stable APOA1 cell line?

How was the stability of APOA1 expression assessed and maintained in this CHO-K1 stable cell line?

Can you provide insights into the characterization of APOA1 expression in the CHO-K1 stable cell line, including its secretion profile and functional implications?

What quality control measures were implemented during the development of this stable cell line?

How does the expression pattern and functional properties of APOA1 in this stable cell line relate to its physiological roles and relevance in disease states?

Stable APOA1 expression.

Secret weapon

Elevated cardiovascular disease research.

Credible findings

Simplified cholesterol homeostasis study

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