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Cat.No. : CSC-RT0095
Host Cell: MDA-MB-231 Target Gene: PIWIL2
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0095 |
| Description | MDA-MB-231-PIWIL2 cell line is a stable cell line with a homozygous knockout of PIWIL2 |
| Target Gene | PIWIL2 |
| Host Cell | MDA-MB-231 |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
I am truly impressed by the effectiveness of PIWIL2 Knockout Cell Line-MDA-MB-231. It has provided me with a powerful tool to investigate gene function with precision and reliability.
United States
07/19/2022
PIWIL2 Knockout Cell Line-MDA-MB-231 has revolutionized my research. Its high efficiency and consistent knockout results have allowed me to uncover crucial insights into the biological pathways and mechanisms I study.
United States
11/03/2022
Using PIWIL2 Knockout Cell Line-MDA-MB-231 has been a outstanding helper for me. Its user-friendly workflow and robust performance have simplified the gene editing process, saving me time and resources.
United Kingdom
02/23/2023
I have tried other gene knockout reagents in the past, but none have matched the performance of PIWIL2 Knockout Cell Line-MDA-MB-231. Its exceptional knockout efficiency and reproducibility make it my top choice.
United States
04/11/2023
PIWIL2 Knockout Cell Line-MDA-MB-231 has opened up new possibilities for my research. Its reliable and efficient knockout capabilities have empowered me to explore gene function in depth and advance my scientific discoveries.
United States
08/23/2020
I cannot overstate the value of PIWIL2 Knockout Cell Line-MDA-MB-231 in my scientific endeavors. Its high efficiency and reproducibility have enabled me to generate robust knockout models for in-depth functional studies.
United States
05/12/2021
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