One-Step EvaGreen qRT-PCR Kit

Cholangiocarcinoma (CCA) presents significant clinical challenges due to its high incidence and late diagnosis, particularly in regions endemic for liver fluke infections. The researchers investigated the regulatory role of serine/arginine-rich splicing factors (SRSFs) in CCA progression, focusing on SRSF1. Using CCA cell lines KKU-055 and KKU-213A, they employed small interfering RNAs to selectively silence SRSF1 and assessed subsequent changes in cell proliferation, apoptosis, and autophagy. RNA extraction, cDNA synthesis, and quantitative PCR were performed to monitor gene expression, while protein abundance and cellular localization of apoptotic and autophagic markers were analyzed by western blotting, immunofluorescence, and transmission electron microscopy. This approach allowed precise evaluation of SRSF1's impact on oncogenic splicing events and cell death pathways, with the Creative Biogene qRT-PCR kit facilitating accurate quantification of target gene transcripts.

Figure 1. Creative Biogene's qRT-PCR reagents enable reliable quantification of gene expression changes, supporting mechanistic studies of apoptosis and autophagy in cancer research. Their application facilitates precise analysis of splicing factor modulation and downstream molecular effects. (Inpad C, et al., 2025)

Silencing SRSF1 in KKU-213A cells induced increased caspase-3 and BAX expression, decreased Bcl-2 levels, and elevated autophagic markers LC3B-II, Beclin-1, and ATG5, as confirmed by western blotting, qPCR, and TEM.