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One-Step EvaGreen qRT-PCR Kit

For research use only. Not intended for any clinical use.
Cat.No.
QEOC-01
Description
One-Step EvaGreen qRT-PCR Kit is a complete qPCR system containing all necessary reagents for both reverse transcription and PCR amplification to occur in a single qPCR reaction tube. This One-Step qRT-PCR kit offers the end-users an efficient, easy to use and reliable alternative to conventional two-step sequential qRT-PCR. Gene-specific primers must be used along with this kit.
Applications
For Real Time PCR Quantification of DNA using EvaGreen
Features
•Sensitivity: one-step mix to amplify both RNA and DNA with high sensitivity
•Efficiency: increased qRT-PCR speed on fast and on standard instruments
•Reliability: formulated to handle common RT-PCR inhibitors
Method
EvaGreen Dye based
Note
Using gene specific primers
Report Gene
EvaGreen and single tube reaction
Sample Type
RNA
Selection Marker
RNA
Sensitivity
Variable
Storage
Store at -20°C and at dark condtion

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Customer Q&As
What is the minimum cDNA needed for qPCR?

A: Optimum performance of qPCR should be in the range of 1-100ng cDNA/20μL reaction mixture. But it does vary depending on the gene of interest (e.g. basal gene expression).

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