One-Step SYBR qRT-PCR Kit with ROX (Perfect Real Time) has been developed for fast RT-qPCR and has been validated on several commonly used real-time instruments requiring normalization with ROX. In this kit, RT-PCR can be performed in a single tube, simplifying the protocol and minimizing the risk of contamination. This Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR.
•Rapid hot start capability: saving time and reducing primer-dimer formation •Unique buffer chemistry: for highest specificity and sensitivity •Accurate quantification: from low copy targets •Sensitive: unique buffer chemistry for highest specificity
One-step qRT-PCR using SYBR Green I
SYBR Green I Dye based
One-step real-time RT-PCR
Using gene specific primers.The ROX Reference Dye is supplied for normalization of fluorescent signal intensities within wells when used with real-time PCR instruments which have this option.
Store at -20°C and at dark condtion
For Research Use Only. Not for use in diagnostic procedures.