One-Step SYBR qRT-PCR Kit (with ROX)

Name: One-Step SYBR qRT-PCR Kit (with ROX)
SKU: QSOC-02
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : QSOC-02

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Product Information

Cat. No.	QSOC-02
Description	One-Step SYBR qRT-PCR Kit with ROX (Perfect Real Time) has been developed for fast RT-qPCR and has been validated on several commonly used real-time instruments requiring normalization with ROX. In this kit, RT-PCR can be performed in a single tube, simplifying the protocol and minimizing the risk of contamination. This Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR.
Sample Type	RNA
Report Gene	SYBR and single tube reaction with ROX
Features	•Rapid hot start capability: saving time and reducing primer-dimer formation •Unique buffer chemistry: for highest specificity and sensitivity •Accurate quantification: from low copy targets •Sensitive: unique buffer chemistry for highest specificity
Applications	One-step qRT-PCR using SYBR Green I
Selection Marker	RNA
Sensitivity	Variable
Method	SYBR Green I Dye based
Note	Using gene specific primers.The ROX Reference Dye is supplied for normalization of fluorescent signal intensities within wells when used with real-time PCR instruments which have this option.
Storage	Store at -20°C and at dark condtion

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What is the primer concentration for qPCR SYBR Green?

A: A final concentration of 200 nM for each primer is effective for most reactions. Optimal results may require titrating primer concentrations between 100 and 500 nM.

What is Rox used for in qPCR?

A: ROX (carboxy-X-rhodamine) is used as a passive reference dye in ROX-dependent real-time PCR systems to normalize for differences in fluorescence levels that may occur primarily due to light path changes between wells.

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