Two-Step SYBR qRT-PCR Kit (with ROX)

Name: Two-Step SYBR qRT-PCR Kit (with ROX)
SKU: QSTC-02
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : QSTC-02

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Product Information

Cat. No.	QSTC-02
Description	This Two Step qRT-PCR Kit (With ROX) has all the components required for Real-Time qRT-PCR. In the reverse transcription reaction, it is much more easier to obtain the full-length cDNA. This kit also has high reverse transcription efficiency and high affinity which can be applied to RT reaction for small amount of RNA template and GC-rich template or template of complicated secondary structure. The SYBR Green I can combine with all the dsDNA and can be applied to the detection of many kinds of target sequence. The kit can be applied to a wide linear range and can exactly quantitate the target gene requiring normalization with ROX.
Sample Type	DNA
Report Gene	SYBR and two tube reaction with ROX
Features	•Unique reverse transcriptase: high affinity to RNA and high compatibility with the downstream qPCR •High sensitivity: especially for low abundance expression gene samples
Applications	For Real Time PCR Quantification of DNA using SYBR Green I
Selection Marker	DNA
Sensitivity	Variable
Method	SYBR Green I Dye based
Note	The ROX Reference Dye is supplied for normalization of fluorescent signal intensities within wells when used with real-time PCR instruments which have this option.
Storage	Store at -20°C and at dark condtion

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Customer Q&As

Why is SYBR Green useful in real time PCR?

A: Because SYBR green only binds to double-stranded DNA molecules and not to single-stranded DNA molecules, PCR product concentration can be recorded at each cycle, resulting in real-time amplification curves suitable for automated threshold analysis and quantification.

Is SYBR Green light sensitive?

A: SYBR Green I dye (present in the master mix) is photosensitive. Solutions containing master mixes should be protected from light as much as possible.

What is the wavelength of Rox fluorophore?

A: ROX (carboxy-X-rhodamine) is a fluorescent compound with an excitation peak at 578 nm and an emission peak at 604 nm.

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