Two-Step SYBR qRT-PCR Kit (with ROX)

QSTC-02

This Two Step qRT-PCR Kit (With ROX) has all the components required for Real-Time qRT-PCR. In the reverse transcription reaction, it is much more easier to obtain the full-length cDNA. This kit also has high reverse transcription efficiency and high affinity which can be applied to RT reaction for small amount of RNA template and GC-rich template or template of complicated secondary structure. The SYBR Green I can combine with all the dsDNA and can be applied to the detection of many kinds of target sequence. The kit can be applied to a wide linear range and can exactly quantitate the target gene requiring normalization with ROX.

For Real Time PCR Quantification of DNA using SYBR Green I

•Unique reverse transcriptase: high affinity to RNA and high compatibility with the downstream qPCR
•High sensitivity: especially for low abundance expression gene samples

SYBR Green I Dye based

The ROX Reference Dye is supplied for normalization of fluorescent signal intensities within wells when used with real-time PCR instruments which have this option.

SYBR and two tube reaction with ROX

DNA

DNA

Variable

Store at -20°C and at dark condtion