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SYBR qRT-PCR DimerEraser Kit

For research use only. Not intended for any clinical use.
Cat.No.
QSQC-01
Description
SYBR qRT-PCR DimerEraser Kit is a real-time PCR specific reagent using the SYBR Green I intercalater method.
This product has an improved buffer system which results in improved reaction specificity. It effectively depresses primer dimers, which is especially important in SYBR Green Assay. This kit is possible to measure accurate quantitative analysis over a broad range of template concentration by suppression of non-specific amplification and more sensitive detection.


Applications
For Real Time PCR Quantification of DNA using SYBR Green I
Features
•Quick and accurate detection and quantification of target gene through real time PCR
•Easy-to-use: Just adding PCR primers, template and dH2O to start the reaction
•High amplification efficiency and highly sensitive detection
•The addition of the accessory protein: strongly suppressing mis-priming of primers during PCR reaction and nonspecific amplification
Method
SYBR Green I Dye based
Report Gene
Suppresses mis-priming
Sample Type
DNA
Selection Marker
DNA
Sensitivity
Variable
Storage
Store at -20°C and at dark condtion.

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Q & A

Customer Reviews

Customer Q&As
What is the maximum amplicon length for SYBR Green?

A: RT-PCR amplicons should be between 100-150 bp in length and should not exceed 500 bp.

What wavelength does SYBR Green absorb?

A: SYBR Green is a green fluorescent cyanine dye that absorbs approximately 497 nm and emits fluorescence at approximately 520 nm.

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