Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : QEQC-01
| Cat. No. | QEQC-01 |
| Description |
EvaGreen qRT-PCR Kit is a specially designed for real-time PCR with intercalator method. This product provides a newly developed buffer which provides superior specificity, increased amplification efficiency and high aptitude for high-speed real time PCR. Accordingly, a successful real time PCR is promised with high sensitivity, wide dynamic range and accurate quantification. Gene-specific primers must be used along with this kit.The chemically modified HotStart Taq polymerase, included in EvaGreen qPCR MasterMix, significantly reduces non-specific PCR amplification observed with regular Taq polymerase |
| Sample Type | DNA |
| Report Gene | EvaGreen qPCR |
| Features |
•High sensitivity •Wide dynamic range •Accurate quantification |
| Applications | For Real Time PCR Quantification of DNA using EvaGreen |
| Selection Marker | DNA |
| Sensitivity | Variable |
| Method | EvaGreen Dye based |
| Storage | Store at -20°C and at dark condtion |
A: EvaGreen qPCR is a type of quantitative polymerase chain reaction (qPCR) technique that uses the fluorescent dye EvaGreen to monitor the amplification of DNA during the PCR process. EvaGreen is a DNA-binding dye that becomes fluorescent when bound to double-stranded DNA.
A: SYBR Green is a DNA-binding dye while EvaGreen is a DNA-binding dye with a longer wavelength of excitation and emission. Both dyes intercalate into double-stranded DNA, enabling fluorescence signal generation.
A: EvaGreen qPCR offers advantages such as sensitivity, specificity, and cost-effectiveness compared to other qPCR techniques that use different fluorescent dyes or probes.
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