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EvaGreen qRT-PCR Kit

For research use only. Not intended for any clinical use.
Cat.No.
QEQC-01
Description
EvaGreen qRT-PCR Kit is a specially designed for real-time PCR with intercalator method. This product provides a newly developed buffer which provides superior specificity, increased amplification efficiency and high aptitude for high-speed real time PCR. Accordingly, a successful real time PCR is promised with high sensitivity, wide dynamic range and accurate quantification. Gene-specific primers must be used along with this kit.The chemically modified HotStart Taq polymerase, included in EvaGreen qPCR MasterMix, significantly reduces non-specific PCR amplification observed with regular Taq polymerase
Applications
For Real Time PCR Quantification of DNA using EvaGreen
Features
•High sensitivity
•Wide dynamic range
•Accurate quantification
Method
EvaGreen Dye based
Report Gene
EvaGreen qPCR
Sample Type
DNA
Selection Marker
DNA
Sensitivity
Variable
Storage
Store at -20°C and at dark condtion

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Q & A

Customer Reviews

Customer Q&As
What is EvaGreen qPCR?

A: EvaGreen qPCR is a type of quantitative polymerase chain reaction (qPCR) technique that uses the fluorescent dye EvaGreen to monitor the amplification of DNA during the PCR process. EvaGreen is a DNA-binding dye that becomes fluorescent when bound to double-stranded DNA.

What is the difference between SYBR Green and EvaGreen?

A: SYBR Green is a DNA-binding dye while EvaGreen is a DNA-binding dye with a longer wavelength of excitation and emission. Both dyes intercalate into double-stranded DNA, enabling fluorescence signal generation.

What are the advantages of EvaGreen PCR?

A: EvaGreen qPCR offers advantages such as sensitivity, specificity, and cost-effectiveness compared to other qPCR techniques that use different fluorescent dyes or probes.

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