SYBR qRT-PCR Kit (with ROX)

Name: SYBR qRT-PCR Kit (with ROX)
SKU: QSQC-02
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : QSQC-02

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Product Information

Cat. No.	QSQC-02
Description	SYBR qRT-PCR Kit ( with ROX ) has been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time instruments requiring normalization with ROX. A combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensures the produces fast, highly-specific and ultra-sensitive real-time PCR.
Sample Type	DNA
Report Gene	SYBR qPCR with ROX
Features	•Rapid: saving time and reducing primer-dimer formation •Unique buffer chemistry: for highest specificity •Broad dynamic range: For high sensitivity •Simple and reproducible: just adding primers and template
Applications	For Real Time PCR Quantification of DNA using SYBR Green I
Selection Marker	DNA
Sensitivity	Variable
Method	SYBR Green I Dye based
Note	The ROX Reference Dye is supplied for normalization of fluorescent signal intensities within wells when used with real-time PCR instruments which have this option.
Storage	Store at -20°C and at dark condtion

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How does SYBR Green work in qPCR?

A: SYBR Green is a free-floating fluorescent dye that binds to double-stranded DNA and increases in fluorescence when bound.

What is the point of ROX in qPCR?

A: Since ROX is a passive fluorescent dye, the main reason to use it in qPCR is for signal normalization. By normalizing the fluorescence signal, variability between technical replicates can be reduced.

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