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SYBR qRT-PCR Kit (with ROX)

For research use only. Not intended for any clinical use.
Cat.No.
QSQC-02
Description
SYBR qRT-PCR Kit ( with ROX ) has been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time instruments requiring normalization with ROX. A combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensures the produces fast, highly-specific and ultra-sensitive real-time PCR.
Applications
For Real Time PCR Quantification of DNA using SYBR Green I
Features
•Rapid: saving time and reducing primer-dimer formation
•Unique buffer chemistry: for highest specificity
•Broad dynamic range: For high sensitivity
•Simple and reproducible: just adding primers and template
Method
SYBR Green I Dye based
Note
The ROX Reference Dye is supplied for normalization of fluorescent signal intensities within wells when used with real-time PCR instruments which have this option.
Report Gene
SYBR qPCR with ROX
Sample Type
DNA
Selection Marker
DNA
Sensitivity
Variable
Storage
Store at -20°C and at dark condtion

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Q & A

Customer Reviews

Customer Q&As
How does SYBR Green work in qPCR?

A: SYBR Green is a free-floating fluorescent dye that binds to double-stranded DNA and increases in fluorescence when bound.

What is the point of ROX in qPCR?

A: Since ROX is a passive fluorescent dye, the main reason to use it in qPCR is for signal normalization. By normalizing the fluorescence signal, variability between technical replicates can be reduced.

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