Two-Step SYBR qRT-PCR Kit

Name: Two-Step SYBR qRT-PCR Kit
SKU: QSTC-01
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : QSTC-01

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Product Information

Cat. No.	QSTC-01
Description	This Two Step qRT-PCR Kit has all the components required for Real-Time qRT-PCR. In the reverse transcription reaction, it is much more easier to obtain the full-length cDNA. This kit also has high reverse transcription efficiency and high affinity which can be applied to RT reaction for small amount of RNA template and GC-rich template or template of complicated secondary structure. The SYBR GreenⅠcan combine with all the dsDNA and can be applied to the detection of many kinds of target sequence. The kit can be applied to a wide linear range and can exactly quantitate the target gene.
Sample Type	DNA
Report Gene	SYBR and two tube reaction
Features	•Unique reverse transcriptase: high affinity to RNA and high compatibility with the downstream qPCR •High sensitivity: especially for low abundance expression gene samples
Applications	For Real Time PCR Quantification of DNA using SYBR Green I
Selection Marker	DNA
Sensitivity	Variable
Method	SYBR Green I Dye based
Storage	Store at -20°C and at dark condtion

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Customer Q&As

Do you need a probe for SYBR Green?

A: No probes are required, which can reduce assay setup and running costs, provided your PCR primers are well designed and your reaction is well characterized.

How does SYBR Green dye bind to DNA?

A: SYBR Green is one of the most commonly used fluorescent dyes in qPCR. It binds to double-stranded DNA molecules by intercalating between the DNA bases. Once embedded in DNA, SYBR Green's mobility decreases, causing its energy to be released in the form of fluorescence.

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