BstZI

Name: BstZI
SKU: EROT0306

For research use only. Not intended for any clinical use.

Cat.No.

EROT0306

Description

Bacillus stearothermophilus.

Concentration

10 u/μl

Reaction Conditions

Buffer D. 50 °C. (BstZI exhibits 50% activity at 37 °C.)

Recognition Site

C↓GGCCG

Size/Form

500 units

Storage

10 mM Tris-HCl (pH 7.4), 500 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.5 mg/ml BSA, 0.1% Triton® X-100, 50% glycerol.

Publications

Q & A

Customer Reviews

Customer Q&As

In which buffer system does BstZI restriction enzyme exhibit the highest activity?

A: BstZI's highest activity is typically in a dedicated buffer system containing a high concentration of magnesium ions and an appropriate pH value. The specific optimal buffer conditions should be referred to the instructions provided by the enzyme supplier.

If the cutting efficiency of BstZI restriction enzyme is not ideal, how can experimental conditions be adjusted?

A: Try increasing the amount of enzyme, extending digestion time, or optimizing DNA quality and purity, while ensuring that the components and conditions of the buffer system meet BstZI's requirements.

How can the recovery rate of DNA fragments after BstZI restriction enzyme digestion be improved?

A: After separation by gel electrophoresis, gel cutting recovery techniques or specialized DNA recovery kits can be used to improve the recovery rate of DNA fragments.

How to avoid overlap of adjacent site digestion products when BstZI restriction enzyme cuts at multiple sites?

A: Choose an appropriate length between enzyme cutting sites to avoid generating overlapping fragments; if unavoidable, separate and identify overlapping fragments through gel purification and fragment length analysis.

When using BstZI restriction enzyme in cloning, how to ensure the correct orientation of the target gene fragment?

A: Design directionally cloning compatible restriction sites to ensure that the gene fragment can only be inserted in the correct orientation; alternatively, use vectors with two different restriction sites so that the fragment can only connect to both sites in the correct orientation.

The cutting efficiency of BstZI restriction enzyme on PCR products is low, what is the solution?

A: During PCR amplification, add extra sequences outside the enzyme cutting site to ensure BstZI can effectively recognize and cut; also, ensure that PCR products are purified without residuals from the PCR reaction to avoid affecting the enzyme's cutting efficiency.

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