Pages
Products

BsiHKC I

For research use only. Not intended for any clinical use.
Cat.No.
EROT0272
Reaction Buffer
10 mM Tris-HCl (pH 7.5 at 37 °C)50 mM NaCl10 mM MgCl21 mM DTT100 μg/ml bovine serum albumin
Reaction Conditions
Incubation at 65 °C
Recognition Site
C↓PyCGPuG
Size/Form
2000 Units;500 Units
Source
Bacillus stearothermophilus HKC
Storage
10 mM Tris-HCl (pH 7.5 at 4 °C)50 mM KCl1 mM dithiothreitol0.1 mM EDTA500 μg/ml bovine serum albumin50% (v/v) glycerol
Unit Definition
One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour in a total reaction volume of 50 μl.

Publications

Q & A

Customer Reviews

Customer Q&As
For BsiHKC I, how is the usage defined with 2000 units and 500 units?

A: One unit is defined as the amount of enzyme required to completely digest 1 μg of λ DNA in a total reaction volume of 50 μl within 1 hour at 65°C.

What is the recognition enzyme cutting site for BsiHKC I? Can the reaction be completed at 37°C?

A: BsiHKC I recognizes the C↓PyCGPuG site and works best at 65°C but does not function at 37°C.

What buffer is required for BsiHKC I?

A: BsiHKC I is supplied with CMX Buffer 3, which provides the highest activity. CMX Buffer 2 or CMX Buffer 4 can be used as alternatives if CMX Buffer 3 is not available, but the effect is not as good as with Buffer 3.

What is the composition of the provided CMX Buffer 3 for BsiHKC I?

A: CMX Buffer 3 for BsiHKC I contains 10 mM Tris-HCl (pH 7.5 at 37°C), 50 mM NaCl, 10 mM MgCl, 21 mM DTT, and 100 μg/ml bovine serum albumin (BSA).

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

CBpromise

Our promise to you:
Guaranteed product quality, expert customer support.

24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Quick Inquiry

Inquiry