BsiHKC I

Name: BsiHKC I
SKU: EROT0272
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EROT0272

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Product Information

Cat. No.	EROT0272
Source	Bacillus stearothermophilus HKC
Recognition Site	C↓PyCGPuG
Size	2000 Units; 500 Units
Unit Definition	One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour in a total reaction volume of 50 μl.
Reaction Buffer	10 mM Tris-HCl (pH 7.5 at 37 °C)50 mM NaCl10 mM MgCl21 mM DTT100 μg/ml bovine serum albumin
Reaction Conditions	Incubation at 65 °C
Storage	10 mM Tris-HCl (pH 7.5 at 4 °C)50 mM KCl1 mM dithiothreitol0.1 mM EDTA500 μg/ml bovine serum albumin50% (v/v) glycerol

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Customer Q&As

For BsiHKC I, how is the usage defined with 2000 units and 500 units?

A: One unit is defined as the amount of enzyme required to completely digest 1 μg of λ DNA in a total reaction volume of 50 μl within 1 hour at 65°C.

What is the recognition enzyme cutting site for BsiHKC I? Can the reaction be completed at 37°C?

A: BsiHKC I recognizes the C↓PyCGPuG site and works best at 65°C but does not function at 37°C.

What buffer is required for BsiHKC I?

A: BsiHKC I is supplied with CMX Buffer 3, which provides the highest activity. CMX Buffer 2 or CMX Buffer 4 can be used as alternatives if CMX Buffer 3 is not available, but the effect is not as good as with Buffer 3.

What is the composition of the provided CMX Buffer 3 for BsiHKC I?

A: CMX Buffer 3 for BsiHKC I contains 10 mM Tris-HCl (pH 7.5 at 37°C), 50 mM NaCl, 10 mM MgCl, 21 mM DTT, and 100 μg/ml bovine serum albumin (BSA).

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