Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EROT0244
| Cat. No. | EROT0244 |
| Recognition Site | A↓CTATG |
| Size | 400 Units;800 Units |
| Reaction Buffer | 33mM Tris-acetate (pH7.9 at 37 °C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA |
| Reaction Conditions | Incubate at 37 °C |
| Storage | 10mM Tris-HCl (pH 7.5 at 25 °C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol |
A: BcuI recognizes the A^CTAGT site and is not sensitive to Dam methylation, Dcm methylation, or CpG methylation.
A: BcuI is supplied with 10X Buffer and does not require additional preparation. The buffer consists of 33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate, and 0.1 mg/mL BSA.
A: BcuI is available in 400 units and 800 units sizes, and it is recommended to purchase the 800 units size for experiments with high demand.
A: BcuI shares its recognition site A^CTAGT with isoschizomers AhlI and SpeI, and it shows the best cutting efficiency at 37°C.
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