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BcuI

For research use only. Not intended for any clinical use.

Cat. No. :   EROT0244

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Product Information

Cat. No. EROT0244
Recognition Site A↓CTATG
Size 400 Units;800 Units
Reaction Buffer 33mM Tris-acetate (pH7.9 at 37 °C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
Reaction Conditions Incubate at 37 °C
Storage 10mM Tris-HCl (pH 7.5 at 25 °C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol
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Customer Q&As
What is the cutting site for BcuI, and is it sensitive to methylation?

A: BcuI recognizes the A^CTAGT site and is not sensitive to Dam methylation, Dcm methylation, or CpG methylation.

Does BcuI require preparing its own buffer, or is an appropriate buffer provided?

A: BcuI is supplied with 10X Buffer and does not require additional preparation. The buffer consists of 33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate, and 0.1 mg/mL BSA.

Is there a high demand for BcuI, and what is the typical purchase size?

A: BcuI is available in 400 units and 800 units sizes, and it is recommended to purchase the 800 units size for experiments with high demand.

Does BcuI recognize the A^CTAGT site, and can it be recognized by other enzymes?

A: BcuI shares its recognition site A^CTAGT with isoschizomers AhlI and SpeI, and it shows the best cutting efficiency at 37°C.

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