Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EROT0443
| Cat. No. | EROT0443 |
| Recognition Site | A↓CATGT |
| Size | 200 Units;400 Units |
| Reaction Buffer | 33mM Tris-acetate (pH7.9 at 37 °C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA |
| Reaction Conditions | Incubate at 37 °C |
| Storage | 10mM Tris-HCl (pH7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol |
A: PscI is at a concentration of 10U/μl, so a total of 200U is 20μl.
A: PscI restriction endonuclease recognizes the A^CATGT site.
A: The cutting efficiency of PscI enzyme is optimal at 37°C in Tango buffer.
A: PscI boasts excellent quality - rigorous quality control and industry-leading production processes, convenient color-coded five-buffer systems, including a universal Tango buffer for double digestion, and multiple options for specific restriction nucleases.
A: The optimal DNA concentration range in a restriction digestion mixture is 0.02-0.1 μg/μL. We recommend that the volume of DNA samples should not exceed 30% of the total reaction volume to avoid potential reaction inhibition.
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