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PscI

For research use only. Not intended for any clinical use.
Cat.No.
EROT0443
Reaction Buffer
33mM Tris-acetate (pH7.9 at 37 °C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
Reaction Conditions
Incubate at 37 °C
Recognition Site
A↓CATGT
Size/Form
200 Units;400 Units
Storage
10mM Tris-HCl (pH7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

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Q & A

Customer Reviews

Customer Q&As
What is the volume in a 200 U package?

A: PscI is at a concentration of 10U/μl, so a total of 200U is 20μl.

What is the recognition site for PscI?

A: PscI restriction endonuclease recognizes the A^CATGT site.

Under what conditions does PscI exhibit optimal cutting efficiency?

A: The cutting efficiency of PscI enzyme is optimal at 37°C in Tango buffer.

What are the advantages and characteristics of PscI?

A: PscI boasts excellent quality - rigorous quality control and industry-leading production processes, convenient color-coded five-buffer systems, including a universal Tango buffer for double digestion, and multiple options for specific restriction nucleases.

What is the optimal DNA concentration for digestion with PscI?

A: The optimal DNA concentration range in a restriction digestion mixture is 0.02-0.1 μg/μL. We recommend that the volume of DNA samples should not exceed 30% of the total reaction volume to avoid potential reaction inhibition.

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