Cat.No. : CSC-RO0689 Host Cell: MB49
| Cat. No. | CSC-RO0689 |
| Description | This cell line is engineered to stably overexpress mouse fibroblast activation protein (Fap) in MB49. |
| Gene | Fap |
| Host Cell | MB49 |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Synthetic peptidolipopolymer promotes specific binding to bladder tumor cell fibronectin (FBN) matrix, enhancing cellular internalization of FBN-integrin complexes via liposomes. Researchers demonstrate the efficacy of FAP Stable Cell Line - MB49 in targeting bladder tumor cells. The synthetic peptidolipopolymer conjugate, when incorporated into liposomes, binds specifically to the fibronectin matrix surrounding bladder tumor cells, enhancing cellular internalization. The peptide shows sequence-specific and concentration-dependent association with MB49 cells, with optimal binding at 2 mol% RWFV-PEG2000-DSPE. Double PEGylation of liposomes enhances binding by > 1.6-fold. Peptide-lipopolymer construct specificity is confirmed by comparing liposomes containing RWFV-PEG2000-DSPE with scrambled and non-peptidic formulations. In MB49 tumor-bearing mice, FAP peptide-targeted liposomes exhibit greater radiance values in tumor-associated regions, indicating potential for targeted delivery in vivo.
Figure 1. In vivo bladder tumor tissue targeting evaluation was conducted using Fap Stable Cell Line - MB49, comparing liposomes with different functionalized lipopolymers (non-targeted, scrambled, or targeted) versus untreated controls.
A: The rationale for using the MB49 cell line likely involves its relevance to cancer research, particularly in bladder cancer studies, where Fap expression and function are of interest.
A: Stability was confirmed through methods such as immunoblotting, flow cytometry, or qPCR, with continuous selection pressure applied to ensure maintenance of Fap expression.
A: Characterization may involve immunofluorescence analysis to determine Fap localization and functional assays to assess its role in tumor progression, stromal remodeling, or immune modulation.
A: Quality control measures likely included screening for mycoplasma contamination, confirmation of stable transgene integration, and assessment of phenotypic stability and consistency.
A: Comparative analysis with in vivo models or clinical data helps validate the relevance of Fap expression in tumor biology and its potential as a therapeutic target or prognostic marker in cancer.
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Using this Mouse Fap Stable Cell Line feels like having a superpower! Its robust Fap expression makes studying fibroblast activation a breeze.
Can't believe how much easier my research has become with this Mouse Fap Stable Cell Line! Its reliable Fap expression has made studying fibrosis pathways a joy.
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Huge shoutout to this Mouse Fap Stable Cell Line! Its strong Fap expression has simplified my experiments and made studying fibrosis mechanisms a lot less stressful.
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