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DAAM1


Official Full Name
dishevelled associated activator of morphogenesis 1
Organism
Homo sapiens
Gene ID
23002
Background
Cell motility, adhesion, cytokinesis, and other functions of the cell cortex are mediated by reorganization of the actin cytoskeleton and several formin homology (FH) proteins have been associated with these processes. The protein encoded by this gene contains two FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. A key regulator of cytoskeletal architecture, the small GTPase Rho, is activated during development by Wnt/Fz signaling to control cell polarity and movement. The protein encoded by this gene is thought to function as a scaffolding protein for the Wnt-induced assembly of a disheveled (Dvl)-Rho complex. This protein also promotes the nucleation and elongation of new actin filaments and regulates cell growth through the stabilization of microtubules. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2012]
Synonyms
ERR3; ERRg; NR3B3; ERRgamma; ERR-gamma

Cat.No. Product Name Price
SHH274293 shRNA set against Human DAAM1 (NM_014992.2) Inquiry
SHH274301 shRNA set against Rat DAAM1 (NM_001108030.1) Inquiry
SHG220463 shRNA set against Mouse Daam1(NM_172464.2) Inquiry
SHG220517 shRNA set against Mouse Daam1(NM_026102.2) Inquiry
SHH274297 shRNA set against Mouse DAAM1 (NM_172464.2) Inquiry
SHH287473 shRNA set against Human ESRRG (NM_001438.3) Inquiry
SHH287477 shRNA set against Mouse ESRRG (NM_011935.3) Inquiry
SHH287481 shRNA set against Rat ESRRG (NM_203336.2) Inquiry
SHW000674 shRNA set against Chicken ESRRG (NM_001007081) Inquiry
SHW003064 shRNA set against Chicken DAAM1 (NM_001193624) Inquiry
Cat.No. Product Name Price
CDCB162149 Chicken ESRRG ORF Clone (NM_001007081) Inquiry
MiUTR1M-03669 DAAM1 miRNA 3'UTR clone Inquiry
MiUTR1M-03668 DAAM1 miRNA 3'UTR clone Inquiry
CDFR007701 Rat Daam1 cDNA Clone(NM_001108030.1) Inquiry
MiUTR3H-08618 DAAM1 miRNA 3'UTR clone Inquiry
MiUTR4H-TG11148 ESRRG miRNA 3'UTR clone Inquiry
CDCR061492 Human ESRRG ORF clone (NM_001243507.1) Inquiry
CDCR061494 Human ESRRG ORF clone (NM_001243506.1) Inquiry
CDCR061496 Human ESRRG ORF clone (NM_001243505.1) Inquiry
CDCR061500 Mouse Esrrg ORF clone (NM_001243792.1) Inquiry
CDCG000573 Mouse ESRRG ORF clone(NM_011935.3) Inquiry
CDCR327570 Human ESRRG ORF Clone(NM_206595.2) Inquiry
CDCR349882 Human ESRRG ORF Clone(NM_001134285.2) Inquiry
CDCR374728 Rat Daam1 ORF Clone(NM_001108030.1) Inquiry
CDCR382388 Rat Esrrg ORF Clone(NM_203336.2) Inquiry
CDCS407990 Human ESRRG ORF Clone (BC064700) Inquiry
CDCB193351 Rabbit DAAM1 ORF clone (XM_002709767.2) Inquiry
CDCB184585 Rabbit ESRRG ORF clone (XM_008268422.1) Inquiry
CDCB164539 Chicken DAAM1 ORF Clone (NM_001193624) Inquiry
CDCR061490 Human ESRRG ORF clone (NM_001243518.1) Inquiry
CDCR258150 Mouse Daam1 ORF Clone(NM_026102.2) Inquiry

Detailed Information

Dishevelled Associated Activator of Morphogenesis 1 (DAAM1) is a pivotal adapter protein, belonging to the Dishevelled-associated activator of morphogenesis (DAAM) family. Much like its counterparts in the Grb7/Gr10/Grb14 protein family, DAAM1 interacts with various receptor tyrosine kinases, exerting profound influence over diverse signaling pathways within the cell.

Expression of DAAM1

Initially identified in multiple tissues, DAAM1 mRNA expression was found to be abundant in skeletal muscle, brain, heart, cartilage, and adipose tissue in mice. Similarly, in humans, DAAM1 exhibited higher expression in the pancreas and skeletal muscle, intermediate levels in the brain and cardiac muscle, and lower expression in tissues such as the liver, kidney, lung, placenta, spleen, prostate, ovary, colon, testis, and small intestine.

Structure of DAAM1

The protein structure of DAAM1 includes various domains such as the pleckstrin homology domain (PH), the N-terminal proline-rich region (PR), a Ras-associating domain (RA), a C-terminal Src homology 2 domain (SH2), and the family-specific BPS domain. These domains are pivotal in defining DAAM1's functions. For example, the SH2 domain allows DAAM1 to interact with phosphorylated tyrosine residues of other proteins, facilitating its role in various signaling pathways. Additionally, the BPS domain has been implicated in DAAM1's involvement in insulin signaling, where it interacts with insulin receptor (IR) and insulin-like growth factor 1 receptor (IGF1R), modulating essential cellular responses. DAAM1, like other methylated proteins, directs the nucleation and elongation of new actin filaments using its conserved methylated homology-2 (FH2) structural domain. It is a Rho-regulated protein that has been linked to the regulation of neuronal development and formation of the stomach. It has been identified as a binding protein for the Wnt receptor-associated protein Disheveled.

Function and Specificities of DAAM1

The multifaceted role of DAAM1 in cellular processes, encompasses cell proliferation, metabolism, and apoptosis. Notably, dysregulation of DAAM1 expression has been linked to specific disease contexts, including certain types of cancers. For instance, upregulation of DAAM1 mRNA has been detected in primary cervical squamous cell cancers. Experimental depletion of DAAM1 mRNA through siRNA techniques resulted in marked inhibition of cell growth in cervical squamous cell carcinoma, underscoring DAAM1's significance as a survival factor in this disease.

DAAM1 participates in the Wnt/PCR Pathway.Figure 1. DAAM1 participates in the Wnt/PCR Pathway.

DAAM1 is a versatile adapter protein crucial in regulating diverse cellular processes and exhibits significant relevance in the context of cancer, specifically cervical squamous cell carcinoma. Its intricate regulatory mechanisms, coupled with its interactions in critical signaling pathways, highlight its importance in both normal cellular functions and disease contexts.

References:

  1. Shi D .Planar cell polarity regulators in asymmetric organogenesis during development and disease[J].Journal of Genetics and Genomics,2023,50(02):63-76.
  2. Ajima, R., Bisson, J.A., Helt, J.C., Nakaya, M.A., Habas, R., Tessarollo, L., He, X., Morrisey, E.E., Yamaguchi, T.P., Cohen, E.D., 2015. DAAM1 and DAAM2 are co-required for myocardial maturation and sarcomere assembly. Dev. Biol. 408, 126-139.
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