Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EROT0317
| Cat. No. | EROT0317 |
| Recognition Site | G↓TAC |
| Size | 1500 Units;3000 Units |
| Reaction Buffer | 10mM Tris-HCl (pH 7.5 at 37 °C), 10mM MgCl2 and 0.1mg/mL BSA |
| Reaction Conditions | Incubate at 37 °C |
| Storage | 10mM potassium-phosphate (pH 7.0 at 25 °C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.5mg/mL BSA and 50% (v/v) glycerol |
A: This enzyme is a restriction endonuclease that recognizes the sequence G↓TAC.
A: Usually, as long as the methylation is not overlapping with the recognition sequence, it would not affect the efficiency of enzyme digestion.
A: Yes, the fragment that has been digested and ligated by this enzyme can still be digested again without affecting its efficiency.
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Even if the fragment is methylated or contains overlapping CpG, it can be successfully cleaved to obtain the desired fragment.
It can be used together with other enzymes for double digestion.
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