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Csp6I

For research use only. Not intended for any clinical use.
Cat.No.
EROT0317
Reaction Buffer
10mM Tris-HCl (pH 7.5 at 37 °C), 10mM MgCl2 and 0.1mg/mL BSA
Reaction Conditions
Incubate at 37 °C
Recognition Site
G↓TAC
Size/Form
1500 Units;3000 Units
Storage
10mM potassium-phosphate (pH 7.0 at 25 °C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.5mg/mL BSA and 50% (v/v) glycerol

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Q & A

Customer Reviews

Customer Q&As
What is the recognition sequence of this enzyme?

A: This enzyme is a restriction endonuclease that recognizes the sequence G↓TAC.

Would the methylation modification on the target fragment affect the enzyme digestion efficiency?

A: Usually, as long as the methylation is not overlapping with the recognition sequence, it would not affect the efficiency of enzyme digestion.

Can this enzyme be used to digest the fragment that has been digested and ligated?

A: Yes, the fragment that has been digested and ligated by this enzyme can still be digested again without affecting its efficiency.

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Customer Reviews
High specificity and strong activity

Even if the fragment is methylated or contains overlapping CpG, it can be successfully cleaved to obtain the desired fragment.

United States

03/31/2023

Strong flexibility

It can be used together with other enzymes for double digestion.

United States

01/02/2021

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