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Csp6I

For research use only. Not intended for any clinical use.

Cat. No. :   EROT0317

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Product Information

Cat. No. EROT0317
Recognition Site G↓TAC
Size 1500 Units;3000 Units
Reaction Buffer 10mM Tris-HCl (pH 7.5 at 37 °C), 10mM MgCl2 and 0.1mg/mL BSA
Reaction Conditions Incubate at 37 °C
Storage 10mM potassium-phosphate (pH 7.0 at 25 °C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.5mg/mL BSA and 50% (v/v) glycerol
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Q & A

Customer Reviews

Customer Q&As
What is the recognition sequence of this enzyme?

A: This enzyme is a restriction endonuclease that recognizes the sequence G↓TAC.

Would the methylation modification on the target fragment affect the enzyme digestion efficiency?

A: Usually, as long as the methylation is not overlapping with the recognition sequence, it would not affect the efficiency of enzyme digestion.

Can this enzyme be used to digest the fragment that has been digested and ligated?

A: Yes, the fragment that has been digested and ligated by this enzyme can still be digested again without affecting its efficiency.

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Customer Reviews
High specificity and strong activity

Even if the fragment is methylated or contains overlapping CpG, it can be successfully cleaved to obtain the desired fragment.

United States

Strong flexibility

It can be used together with other enzymes for double digestion.

United States

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