Hpy8I

Name: Hpy8I
SKU: EROT0368

For research use only. Not intended for any clinical use.

Cat.No.

EROT0368

Reaction Buffer

3mM Tris-acetate (pH 7.9 at 37 °C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA

Reaction Conditions

Incubate at 37 °C

Recognition Site

CTN↓NAC

Size/Form

200 Units;400 Units

Storage

10mM Tris-HCl (pH7.5 at 25 °C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA and 50% (v/v) glycerol

Publications

Q & A

Customer Reviews

Customer Q&As

What is the specific recognition sequence of Hpy8I enzyme?

A: The specific recognition sequence of Hpy8I restriction enzyme is 5'-GTNACC-3'.

What factors are related to the cutting efficiency of Hpy8I?

A: The cutting efficiency of Hpy8I may be affected by factors such as substrate DNA concentration, enzyme concentration, reaction time, reaction temperature, and buffer system.

Among various buffer systems, which one demonstrates the best activity performance for Hpy8I?

A: Different buffer systems may have different impacts on Hpy8I's activity, and the optimal buffer system needs to be determined through experimentation.

Compared to other restriction enzymes, what advantages does Hpy8I have in DNA cloning and genetic engineering?

A: Hpy8I may have advantages in certain applications due to its specific recognition sequence or cutting efficiency, but specific advantages need to be evaluated based on the experimental objectives and conditions.

Are the cutting product ends of Hpy8I overhang, blunt, or sticky?

A: The cutting product ends of Hpy8I are 3' overhangs, meaning, it produces sticky ends.

In which specific molecular biology applications is the use of Hpy8I most common?

A: Hpy8I can be used in DNA cloning, genomic analysis, gene tagging, and other DNA manipulation-related molecular biology applications.

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