Cat.No. : CSC-RO0056 Host Cell: HEK293T
| Cat. No. | CSC-RO0056 |
| Description | This cell line is a stably transfected cell line which expresses human Toll-like receptor 4 (TLR4). |
| Gene | TLR4 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Gene Name | TLR4 toll like receptor 4 [ Homo sapiens (human) ] |
| Gene Symbol | TLR4 |
| Synonyms | TOLL; CD284; TLR-4; ARMD10 |
| Gene ID | 7099 |
| UniProt ID | O00206 |
| Chromosome Location | 9q33.1 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The researchers wanted to control pro-inflammatory responses through the TLR4 signaling pathway, which is required for acute infection prevention and recovery from Gram-negative infections. They focused on the TLR4/MD-2 complex, which detects bacterial LPS. They created novel synthetic glycolipids with a picomolar affinity for TLR4/MD-2 using crystal structure-inspired design, known as disaccharide-based lipid A mimetics (DLAMs). These DLAMs mimic the 3D form of E. coli lipid A and maintain structural stiffness through an α,α-(1↔1)-linked disaccharide scaffold. The researchers used phosphorylation and acylation patterns to tailor TLR4 activation in human and mouse immune cells, lung epithelial cells, and TLR4-transfected HEK293 cells.
Figure 1. The researchers utilized the TLR4 Stable Cell Line-HEK293 to study dose-dependent activation and inhibition of TLR4 signaling. They analyzed responses to various ligands compared to known stimulants and inhibitors, calculating EC50 values. The experiments demonstrated both activation and competitive inhibition of TLR4 signaling using synthetic antagonists, with data consolidated from three independent experiments. (Heine H, et al., 2021)
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After using Human TLR4 Stable Cell Line HEK293, the HEK293 cell line grew rapidly and stably, achieving the expected results.
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