Mouse Tlr4 Knockout Cell Line-RAW264.7

Name: Mouse Tlr4 Knockout Cell Line-RAW264.7
SKU: CSC-RT2802
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2802

Target Gene : Tlr4 Host Cell : RAW264.7

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2802
Description	This cell is a stable cell line with a homozygous knockout of mouse Tlr4 using CRISPR/Cas9.
Target Gene	Tlr4
Host Cell	RAW264.7
Host Cell Species	Mus musculus (Mouse)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Tlr4 toll-like receptor 4 [ Mus musculus ]
Gene Symbol	Tlr4
Synonyms	Lps; Ly87; Ran/M1; Rasl2-8
Gene Description	toll-like receptor 4
Gene ID	21898
Uni Prot ID	Q9QUK6
m RNA Refseq	NM_021297.2
Protein Refseq	NP_067272.1
Chromosome Location	4 C1; 4 33.0 cM
Function	lipopolysaccharide binding; lipopolysaccharide receptor activity; phosphatidylinositol 3-kinase binding; protein binding; receptor activity; transmembrane signaling receptor activity;
Pathway	Activated TLR4 signalling, organism-specific biosystem; Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon, organism-specific biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; HIF-1 signaling pathway, organism-specific biosystem;

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