Cat.No. : CSC-RO0069 Host Cell: CHO-K1
| Cat. No. | CSC-RO0069 |
| Description | This cell line is a stably transfected cell line which expresses human programmed cell death 1 (PDCD1). |
| Background | PDCD1 protein is a cell surface membrane protein of the immunoglobulin superfamily. It is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. |
| Gene | PDCD1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Target | Human |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | PDCD1 programmed cell death 1 [ Homo sapiens (human) ] |
| Gene Symbol | PDCD1 |
| Synonyms | PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1 |
| Gene ID | 5133 |
| UniProt ID | Q15116 |
| Chromosome Location | 2q37.3 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Gradually adapt to serum-free culture or add specific serum-free growth factors and nutritional supplements to promote cell proliferation.
A: Optimize the choice and dilution ratio of antibodies, extend the incubation time with cells, and ensure that the cells remain viable before analysis.
A: Optimize cell lysis conditions, use efficient immunoprecipitation reagents and appropriate antibodies for PDCD1 protein, ensure sufficient incubation time, and gentle washing steps.
A: Optimize the design of siRNA or shRNA to ensure specificity for the PDCD1 gene, and adjust transfection efficiency and dosage.
A: Ensure uniform experimental conditions, including cell density, drug concentration, and duration of action, and perform multiple repetitions to increase the reliability of the data.
A: Check and optimize culture conditions, reduce physical or chemical stress factors, and use cell protectants to minimize apoptosis.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
