Human CFTR Stable Cell Line-HEK293

Name: Human CFTR Stable Cell Line-HEK293
SKU: CSC-RI0003
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0003

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0003
Description	This cell line is engineered to overexpress human CFTR.
Target Gene	CFTR
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuCFTR
Alias	CFTR, ABCC7, CF, ABC35, CFTR/MRP, dJ760C5.1, MRP7, TNR CFTR, CF, ABCC7, TNR-CFTR
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CFTR cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) [ Homo sapiens ]
Gene Symbol	CFTR
Synonyms	CF; MRP7; ABC35; ABCC7; CFTR/MRP; TNR-CFTR; dJ760C5.1
Gene Description	cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
GeneID	1080
Uni ProtID	P13569
mRNA Refseq	NM_000492.3
Protein Refseq	NP_000483.3
Chromosome Location	7q31.2
Function	ATP binding; ATP-binding and phosphorylation-dependent chloride channel activity; PDZ domain binding; channel-conductance-controlling ATPase activity; chloride channel activity; enzyme binding; protein binding;
Pathway	ABC transporters, organism-specific biosystem; ABC transporters, conserved biosystem; ABC-family proteins mediated transport, organism-specific biosystem; Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Gastric acid secretion, organism-specific biosystem; Gastric acid secretion, conserved biosystem;
MIM	602421

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Background

Q & A

Customer Reviews

The cystic fibrosis transmembrane conductance regulator (CFTR) is an ion channel that once phosphorylated by PKA is gated by ATP binding and hydrolysis. When open, this channel allows chloride ion flux. The CFTR gene was cloned and has been of interest as a therapeutic target since the first observation that a nucleotide substitution found in patients with cystic fibrosis encodes for a trafficking-deficient mutant channel termed DeltaF508. To date, at least 136 allelic variants of CFTR have been associated with patients that have cystic fibrosis. The CFTR ion channel is expressed in the respiratory tract, sweat glands, intestinal tissue, and pancreas, and decreased surface expression or function of mutant channels in these tissues is consistent with disease symptoms. To date, specific activators and trafficking modulators that normalize surface expression and function toward wild-type levels have shown promise as therapies for cystic fibrosis.

Customer Q&As

What is CFTR?

A: Cystic Fibrosis Transmembrane conductance Regulator (CFTR) is a protein encoded by the CFTR gene in humans.

Has Human CFTR Stable Cell Line-HEK293 cell line been tested for mycoplasma?

A: The cell line has been screened to confirm the absence of mycoplasma.

What is the host cell line for Human CFTR Stable Cell Line-HEK293?

A: The host cell line for Human CFTR Stable Cell Line-HEK293 is 293HEK-Trex, human embryonic kidney, epithelial-like cells, adherent.

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