Human CFTR Stable Cell Line-CHO

Name: Human CFTR Stable Cell Line-CHO
SKU: CSC-RI0081
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0081

Host Cell : CHO Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0081
Description	This cell line is engineered to overexpress human CFTR.
Background	The human CFTR gene encodes the pore-forming subunit of the cAMP-regulated epithelial chloride channel associated with cystic fibrosis.
Target Gene	CFTR
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuCFTR
Alias	CFTR, ABCC7, CF, ABC35, CFTR/MRP, dJ760C5.1, MRP7, TNR CFTR, CF, ABCC7, TNR-CFTR
Host Cell	CHO
Host Cell Species	Cricetulus griseus (Chinese hamster)
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CFTR cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) [ Homo sapiens ]
Gene Symbol	CFTR
Synonyms	CF; MRP7; ABC35; ABCC7; CFTR/MRP; TNR-CFTR; dJ760C5.1
Gene Description	cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
GeneID	1080
Uni ProtID	P13569
mRNA Refseq	NM_000492.3
Protein Refseq	NP_000483.3
Chromosome Location	7q31.2
Function	ATP binding; ATP-binding and phosphorylation-dependent chloride channel activity; PDZ domain binding; channel-conductance-controlling ATPase activity; chloride channel activity; enzyme binding; protein binding;
Pathway	ABC transporters, organism-specific biosystem; ABC transporters, conserved biosystem; ABC-family proteins mediated transport, organism-specific biosystem; Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Gastric acid secretion, organism-specific biosystem; Gastric acid secretion, conserved biosystem;
MIM	602421

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Q & A

Customer Reviews

Customer Q&As

What gene engineering technology was used to construct the Human CFTR Stable Cell Line-CHO?

A: The cell line was constructed by stably transfecting the human CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) gene into CHO cells, using techniques such as molecular cloning and cell screening.

What are the unique advantages of using the Human CFTR Stable Cell Line-CHO in cystic fibrosis research?

A: As this cell line stably expresses the human CFTR gene, it can simulate CFTR functions in cystic fibrosis patient cells and is suitable for drug screening and pathological mechanism studies.

What factors should be considered when using the Human CFTR Stable Cell Line-CHO for drug screening?

A: Attention should be paid to the impact of drugs on CFTR channel functions, including channel opening and closing kinetics and regulation of chloride ion permeability.

What are the potential uses of the Human CFTR Stable Cell Line-CHO in intestinal disease model research?

A: It can be used to study the function of CFTR in intestinal epithelial cells, especially in research related to digestive system pathology in cystic fibrosis.

How can experimental errors be minimized in biological experiments using the Human CFTR Stable Cell Line-CHO?

A: By strictly controlling experimental conditions including cell density, drug concentration, and timing, as well as replicating experiments to ensure the reliability of results.

What is the application of the Human CFTR Stable Cell Line-CHO in respiratory disease research?

A: As CFTR plays a key role in regulating chloride ion and water balance in airway epithelial cells, this cell line can be used for research on cystic fibrosis and other respiratory diseases.

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