Cftr Knockout Mouse Bone Marrow Macrophages

Name: Cftr Knockout Mouse Bone Marrow Macrophages
SKU: CSC-RK0164
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RK0164

Target Gene : Cftr Host Cell : Bone marrow macrophages

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RK0164
Background	Cystic fibrosis transmembrane conductance regulator (CFTR) is a protein that in humans is encoded by the CFTR gene. CFTR is a ABC transporter-class ion channel that transports chloride and thiocyanate ions across epithelial cell membranes. Mutations of the CFTR gene affect functioning of the chloride ion channels in these cell membranes, leading to cystic fibrosis and congenital absence of the vas deferens.
Target Gene	Cftr
Abbr	MPBMMACs-Cftr Knockout
Alias	CFTR, Abcc7, AW495489
Host Cell	Bone marrow macrophages
Host Cell Species	Mus musculus (Mouse)
Size	>1x10⁶ cells/vial
Validation	Sequencing
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Cftr cystic fibrosis transmembrane conductance regulator [ Mus musculus ]
Gene Symbol	CFTR
Synonyms	Abcc7; AW495489
Gene ID	12638
Uni Prot ID	P26361
m RNA Refseq	NM_021050.2
Protein Refseq	NP_066388.1
Chromosome Location	6 A3; 6 3.1 cM
Function	ATP binding; ATPase activity; ATPase activity, coupled to transmembrane movement of substances; PDZ domain binding; channel-conductance-controlling ATPase activity; chloride channel activity; enzyme binding; hydrolase activity; ion channel activity; nucleoside-triphosphatase activity; nucleotide binding;
Pathway	ABC transporters, organism-specific biosystem; ABC transporters, conserved biosystem; ABC-family proteins mediated transport, organism-specific biosystem; Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Gastric acid secretion, organism-specific biosystem; Gastric acid secretion, conserved biosystem; Pancreatic secretion, organism-specific biosystem; Pancreatic secretion, conserved biosystem; Transmembrane transport of small molecules, organism-specific biosystem;

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Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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