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I-PpoI (Intron-Encoded Endonuclease)

For research use only. Not intended for any clinical use.
Cat.No.
EROT0377
Description
I-PpoI is an intron-encoded endonuclease from Physarum polycephalum. The enzyme is produced from a recombinant E. coli strain.
Concentration
100–200u/μl
Reaction Conditions
I-PpoI Buffer. 37 °C.
Recognition Site
CTCTCTTAA↓GGTAGC
Size/Form
1000 units
Storage
50mM Tris-HCl (pH 7.5), 0.01% NP-40, 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

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Q & A

Customer Reviews

Customer Q&As
What is the nucleic acid recognition sequence of the enzyme?

A: The enzyme is an endonuclease with a recognition sequence of C↓GGCCG.

How should the enzyme be stored?

A: We recommend storing the product at -20°C and burying the enzyme in an ice box when in use.

Can overnight digestion be used when using the enzyme for enzyme cutting?

A: Generally, the enzyme digestion time is 2-3 hours, but overnight digestion is also feasible if time does not permit and does not result in adverse effects.

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Customer Reviews
Vector construction

We successfully used this reagent to cleave the pUC gRNA Shuttle vector and construct the corresponding shuttle vector.

United States

04/06/2023

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