Transfected Stable Cell Lines
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Cat. No. : DSPK-08
| Cat. No. | DSPK-08 |
| Description | Methyl-DNA Sample Prep Kit is designed for DNA library construction of fragmented, Bisulfite treatment DNA samples for next generation sequencing (NGS) platforms of Illumina. The kit is suitable for library preparation of various samples, including gDNA, FFPE DNA, Cell-free DNA and ChIP DNA. In addition, the procedure can accommodate with low input DNA (including that derived from FFPE samples), making it ideal for methylation analysis of precious, limited samples. |
| Sample Type | Fragmented, Bisulfite treatment DNA |
| Features |
• Convenient and fast • High ligation efficiency • Low input, compatible with FFPE samples. • High quality and reliable performance |
| Storage | All components should be stored at -20°C. |
A: Library screening is the process of identifying clones carrying a gene of interest. Screening relies on the unique properties of the clones in the library. A DNA library consists of approximately thousands of clones in the form of plaques or colonies on a plate.
A: DNA methylation is the most widely studied epigenetic modification. DNA methylation occurs predominantly on cytosine bases of CpG dinucleotide motifs in most mammalian cells, and on all cytosine backgrounds in plant genomes.
A: Bisulfite sequencing is a method used in molecular biology to determine the pattern of methylation on a specific DNA strand. This technique converts unmethylated cytosine residues to uracil, while leaving methylated cytosines untouched. By comparing the sequence of the treated DNA to the original sequence, researchers can deduce which cytosines were methylated.
A: Some common applications of methyl-seq include whole genome bisulfite sequencing (WGBS) research, identifying genome-wide methylation of cell-free (cfDNA) in research studies, and hybridization capture for targeted methylation studies.
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Great! Simple, 2-hour workflow.
Workflow design for easy automation
The kit allows both non-barcoded (singleplexed) and barcoded (multiplexed) DNA libraries to be quickly constructed using sub-nanogram input concentrations of DNA.
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