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DNA Library Prep Kit for ssDNA

For research use only. Not intended for any clinical use.
Cat.No.
DSPK-07
Description
ssDNA Sample Prep Kit is designed for DNA library construction of fragmented ssDNA/dsDNA samples for next generation sequencing (NGS) platforms of Illumina. The kit is suitable for library preparation of various samples, including gDNA, FFPE DNA, Cell-free DNA, ChIP DNA, viral DNA and single stranded cDNA.
Features
• Convenient and fast
• High ligation efficiency
• Low input, starting from 10 pg~250 ng DNA
• High quality and reliable performance
Sample Type
DNA
Storage
All components should be stored at -20°C.

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Q & A

Customer Reviews

Customer Q&As
What is the difference between genomic library and DNA library?

A: A genomic library is a collection of clones with the total genomic DNA of an organism. A cDNA library contains only coding sequences; it does not contain introns. Genomic libraries consist of the entire genomic DNA, including non-coding (intronic and regulatory) DNA.

Is the presence of RNA in the starting material a problem?

A: Yes. DNA should be relatively free of RNA since large fractions of RNA will impair end repair and dA tailing, resulting in reduced ligation capabilities.

How is a single strand DNA library prepared?

A: Single-stranded library preparation is performed by ligating a biotinylated adapter oligonucleotide to the 3' ends of heat-denatured DNA. The resulting strands are then immobilized on streptavidin-coated beads and copied with a polymerase.

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Customer Reviews
Higher library yields

Higher library yields compared to other library prep kits.

United States

05/12/2021

Minimize sequence bias

The DNA Library Prep Kit minimizes sequence bias in PCR.

United Kingdom

08/15/2021

Compatible with ssDNA and dsDNA samples

Compatible with ssDNA and dsDNA samples. It is suitable for severely damaged, denatured or degraded DNA samples, and is an ideal choice for the preparation of difficult and precious samples.

United States

10/09/2021

Helpful tool

Great buy! DNA Library Prep Kit for ssDNA is also suitable for double-stranded DNA library preparation, as well as single-and double-stranded mixed DNA library preparation.

United States

01/27/2022

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