Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : DSPK-03
| Cat. No. | DSPK-03 |
| Description | Ultra DNA Sample Prep Kit is designed for DNA library construction of fragmented DNA samples for next generation sequencing (NGS) platforms of Illumina. The kit includes all of the enzymes and buffers for end-preparation, adapter ligation and library amplification. Ultra DNA Sample Prep Kit can be used for construction of PCR-free libraries from 100 ng of high-quality fragmented genomic DNA, or 10 ng of circulating cell-free DNA (cfDNA). |
| Sample Type | Fragmented DNA |
| Features |
• Convenient and fast, the entire protocol can be completed in 2 hours • High quality and reliable performance • High library yield, which can meet variety of experimental needs |
| Storage |
The EDTA TE buffer can be stored at room temperature or 4°C. All other components should be stored at -20°C. |
A: The amount of DNA needed for library preparation can vary depending on the specific protocol or kit being used. Our DNA Sample Prep Kit can be used for the construction of PCR-free libraries from 100 ng of high-quality fragmented genomic DNA, or 10 ng of circulating cell-free DNA (cfDNA).
A: In Sanger sequencing, template DNA and primers are used to incorporate chain-terminating dideoxynucleoside triphosphates (ddNTPs) into DNA strands to initiate the reaction during DNA replication. In Illumina sequencing, reversible terminators are attached to regular deoxynucleoside triphosphates (dNTPs).
A: A DNA library is a collection of DNA fragments that have been cloned into a vector so that researchers can identify and isolate their DNA fragments of interest for further study. There are basically two kinds of libraries: genomic DNA libraries and cDNA libraries.
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The prepared library DNA is sufficient in yield and of high quality.
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