HIFF™ DNA Library Preparation Kit for Illumina

DSPK-05

Hyper DNA Sample Prep Kit is designed for DNA library construction of fragmented DNA samples for next generation sequencing (NGS) platforms of Illumina. The kit includes all of the enzymes and buffers for end-preparation, adapter ligation and library amplification. The kit also provides DNA repair enzymes, which could repair DNA damages such as nicks, gaps, oxidized bases, damaged/blocked 3’ ends, AP sites (apurinic/apyrimidinic sites), and uracil bases. In addition, the Ligase Enzymes and ligation buffer were optimized to further improve the efficiency of adapter ligation. Hyper DNA Sample Prep Kit can be used for construction of PCR-free libraries from 100 ng of high-quality fragmented genomic DNA, or 10 ng of circulating cell-free DNA (cfDNA).

• Convenient and fast, the entire protocol can be completed in 2 hours
• Repair function, adding a unique repair module which makes the success rate of library preparation of degraded FFPE samples is higher
• High efficiency, it improves the efficiency of adapter ligation and library amplification
• High quality and reliable performance
• High library yield, which can meet variety of experimental needs

Fragmented DNA

All components should be stored at -20°C.