Thermostable dUTPase

Name: Thermostable dUTPase
SKU: EMQZ1423
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMQZ1423

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Product Information

Cat. No.	EMQZ1423
Description	Thermostable dUTPase hydrolyzes dUTP to dUMP and pyrophosphate. Thermostable dUTPase maximizes the efficiency of high-fidelity PCR abd removes contaminating dUTP present in PCR reactions and dNTP solutions. It can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product. The dUTPase increase in PCR product yield, length and fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity and yield-sensitive applications such as cloning and subsequent recombinant protein technology, and gene expression analysis (semi-quantitative RT-PCR techniques and real-time PCR analysis), where small differences in product accumulation can have a significant impact on gene expression analysis.
Source	E.coli strain
Concentration	10,000 U/ml
Size	500 U
Unit Definition	One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85°C.
Storage	Store at -20°C

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Customer Q&As

How is the activity of the Thermostable dUTPase defined?

A: In the buffer system of 20mM Hepes pH 7.5, 150mM KCl, and 5mM MgCl2 at 85°C, the amount of enzyme required to convert 1 μM dUTP to dUMP within 1 minute is defined as 1 unit.

Is the quality guaranteed?

A: The Thermostable dUTPase has undergone multiple column purifications, and SDS-PAGE electrophoresis detects only a clear, single target band.

Can Thermostable dUTPase increase the length of amplification?

A: The addition of dUTPase promotes the amplification of long fragments.

What is Thermostable dUTPase's application?

A: Thermostable dUTPase can increase the yield of PCR amplification and improve the efficiency of long fragment amplification.

Any usage advice?

A: The heat-resistant dUTPase can be directly added to the PCR reaction mixture to enhance PCR performance. The recommended concentration is 10 units of dUTPase per 50 μl reaction system.

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