Single-Stranded DNA Binding Protein

Name: Single-Stranded DNA Binding Protein
SKU: EMOT1130
Rating: 4.0 (3 reviews)

For research use only. Not intended for any clinical use.

Cat.No.

EMOT1130

Description

Single-stranded specific DNA binding protein.Helix destabilizing protein.Ultrapure recombinant protein.

Applications

Reduces formation of secondary DNA structures.Prevents degradation of single stranded DNA by nucleases.Prevents inhibition of PCR by template DNA contaminants.Improves efficiency of DNA amplification by Taq DNA Polymerase.Replaces Hot Start method by assembling PCR reactions.Stabilizes single-stranded regions of DNA for site-specific mutagenesis.Aids completion of restriction enzyme digestion.Improves efficiency of DNA synthesis by T4 DNA Polymerase.Enhances fidelity of modified T4 DNA Polymerase.

Size/Form

500μg;100μg

Source

Escherichia coli

Storage

20 mM Tris-HCl (pH 7.8 at 22 °C)300 mM NaCl5 mM β-mercaptoethanol0.05% Igepal0.2 mM EDTA50% (v/v) glycerol

Publications

Q & A

Customer Reviews

Customer Q&As

Does it help if some target fragments can't be amplified?

A: You can try using the Single-Stranded DNA Binding Protein, which has been shown to improve PCR efficiency on difficult templates by increasing the polymerases' processivity and their affinity for the primer-template.

What is the efficiency of PCR after adding this protein?

A: The efficiency of PCR can be significantly increased, as the Single-Stranded DNA Binding Protein can minimize missing mutations in the PCR product, thereby increasing the yield.

What is the purity of this protein?

A: After SDS-PAGE analysis, the purity of the reagent is greater than 95%, and it does not contain nonspecific endonucleases, exonucleases, or RNases.

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Customer Reviews

High replication efficiency

Single-strand DNA Binding Protein binds to single-stranded DNA with high affinity, which makes the helix unstable and allows DNA polymerase to more easily access the substrate, thereby improving DNA replication efficiency.

United States

01/12/2020

Site-directed mutagenesis

Single-stranded DNA Binding Protein can be used with RecA protein for site-directed mutagenesis and sequence selection from double-stranded DNA libraries.

United States

04/11/2021

SNP analysis

Single-Stranded DNA Binding Protein was used to help obtain longer read lengths in pyrosequencing for SNP analysis.

United States

11/21/2021

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Single-Stranded DNA Binding Protein

Publications

Q & A

Customer Reviews

Does it help if some target fragments can't be amplified?

What is the efficiency of PCR after adding this protein?

What is the purity of this protein?

High replication efficiency

Site-directed mutagenesis

SNP analysis

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