Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EMOT1130
| Cat. No. | EMOT1130 |
| Description | Single-stranded specific DNA binding protein.Helix destabilizing protein.Ultrapure recombinant protein. |
| Source | Escherichia coli |
| Applications | Reduces formation of secondary DNA structures.Prevents degradation of single stranded DNA by nucleases.Prevents inhibition of PCR by template DNA contaminants.Improves efficiency of DNA amplification by Taq DNA Polymerase.Replaces Hot Start method by assembling PCR reactions.Stabilizes single-stranded regions of DNA for site-specific mutagenesis.Aids completion of restriction enzyme digestion.Improves efficiency of DNA synthesis by T4 DNA Polymerase.Enhances fidelity of modified T4 DNA Polymerase. |
| Size | 500μg;100μg |
| Storage | 20 mM Tris-HCl (pH 7.8 at 22 °C)300 mM NaCl5 mM β-mercaptoethanol0.05% Igepal0.2 mM EDTA50% (v/v) glycerol |
A: You can try using the Single-Stranded DNA Binding Protein, which has been shown to improve PCR efficiency on difficult templates by increasing the polymerases' processivity and their affinity for the primer-template.
A: The efficiency of PCR can be significantly increased, as the Single-Stranded DNA Binding Protein can minimize missing mutations in the PCR product, thereby increasing the yield.
A: After SDS-PAGE analysis, the purity of the reagent is greater than 95%, and it does not contain nonspecific endonucleases, exonucleases, or RNases.
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Single-strand DNA Binding Protein binds to single-stranded DNA with high affinity, which makes the helix unstable and allows DNA polymerase to more easily access the substrate, thereby improving DNA replication efficiency.
Single-stranded DNA Binding Protein can be used with RecA protein for site-directed mutagenesis and sequence selection from double-stranded DNA libraries.
Single-Stranded DNA Binding Protein was used to help obtain longer read lengths in pyrosequencing for SNP analysis.
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