RobustCutter Sumo Protease

Name: RobustCutter Sumo Protease
SKU: EMQZ1420
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMQZ1420

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Product Information

Cat. No.	EMQZ1420
Description	SUMO Protease, also known as Ulp, is a highly active cysteinyl protease. SUMO Protease cleaves in a highly specific manner and can be used cleave SUMO (Small Ubiquitin-like Modifier) from recombinant fusion proteins. Unlike most proteases, SUMO Protease recognizing the tertiary structure of the ubiquitin-like (UBL) protein instead of an amino acid sequence. The optimal temperature for cleavage is 30°C; however, the enzyme is active over wide ranges of temperature and pH (pH 7.0–9.0). Following digestion, SUMO Protease is easily removed from the cleavage reaction by affinity chromatography using the polyhistidine tag at the N-terminus of the protease.
Source	A recombinant E. coli strain that carries Ulp1 gene fragment from Saccharomyces cerevisiae.
Concentration	2,000 U/ml
Size	200 U
Unit Definition	One unit of SUMO Protease cleaves greater than 90% of 100 ug control substrate (SUMO-eGFP) in 1 h at 30°C.
Reaction Conditions	SUMO Protease Storage: 25 mM Tris-HCl (pH 8.0 at 25°C),1% IGEPAL CA-630 (NP-40), 250 mM NaCl,50 μM DTT,50% (v/v) glycerol.10× SUMO Protease Buffer (Salt plus):500 mM Tris-HCl (pH 8.0 at 25°C),2% IGEPAL CA-630 (NP-40),1.5 M NaCl,10 mM DTT.10× SUMO Protease Buffer (Salt free):500 mM Tris-HCl (pH 8.0 at 25°C),2% IGEPAL CA-630 (NP-40),10 mM DTT.
Storage	Store at -20°C

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Customer Q&As

How is the catalytic activity of the Sumo Protease?

A: SUMO Protease has its optimal catalytic temperature at 30°C but exhibits activity over a broad temperature range (4°C to 30°C) and pH range (pH 7.0 to 9.0).

Does the Sumo Protease contain a His-tag?

A: Yes, the SUMO Protease is equipped with a His-tag, which allows for easy removal by affinity chromatography after cleavage of the fusion protein.

Can overnight digestion be performed when using the RobustCutter Sumo Protease?

A: We recommend overnight incubation at 4°C for fusion protein cleavage. However, users can optimize the reaction conditions based on their specific protein of interest.

Is the sample for RobustCutter Sumo Protease digestion purified protein?

A: For the best results, ensure that the sample is partially or fully purified protein.

Is the RobustCutter Sumo Protease shipped at room temperature?

A: No, the RobustCutter Sumo Protease is shipped with dry ice to maintain its activity during transportation.

What reagents are included in the RobustCutter Sumo Protease?

A: The RobustCutter Sumo Protease generally contains two components: SUMO Protease and 10× SUMO Protease Buffer.

How should RobustCutter Sumo Protease be stored and for how long?

A: The two components have different storage requirements: SUMO Protease can be stored for 24 months at -70°C or 6 months at -20°C, avoiding repeated freezing and thawing; 10× SUMO Protease Buffer can be stored at -20°C for 24 months.

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