High Concentration End-Repair Mix

Name: High Concentration End-Repair Mix
SKU: EMOT1116
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMOT1116

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Product Information

Cat. No.	EMOT1116
Description	This high-concentration formulation of the End-Repair Mix is compatible with applications requiring >1 microgram of DNA to be prepared for blunt-end ligation. The mix converts DNA containing damaged or incompatible 5′- and/or 3′-protruding ends to 5′-phosphorylated, blunt-ended DNA. The conversion to blunt-ended DNA is accomplished by exploiting the 5′→ 3′ polymerase and 3′→ 5′ exonuclease activities of T4 DNA Polymerase (P7080L). T4 Polynucleotide Kinase (Y9040L) ensures that the ends of the blunt-ended DNA fragments are 5′-phosphorylated for subsequent ligation by T4 DNA Ligase (L6030-HC-L).
Source	Purified from strains of E. coli that express the recombinant T4 DNA Polymerase, and T4 Polynucleotide Kinase genes, respectively.
Size	75 Reactions
Reaction Buffer	1 mM Tris-HCl500 mM NaCl100 mM MgCl250 mM DTT0.25% Triton-X 100pH 7.5 @ 25 °C
Storage	100 mM KCl10 mM Tris-HCl0.1 mM EDTA1 mM DTT0.1% Triton X-10050% glycerolpH 7.4 at 25 °C

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Customer Reviews

Customer Q&As

What is the minimum amount of DNA required when using this enzyme?

A: The minimum DNA content for using this enzyme is 1ug.

Which enzymes are included in this mixture?

A: The reagent contains T4 DNA polymerase, T4 polynucleotide kinase, and T4 DNA ligase.

What is the reaction buffer?

A: Reaction buffer system: 1 mM Tris-HCl, 500 mM NaCl, 100 mM MgCl2, 50 mM DTT, 0.25% Triton-X 100.

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Customer Reviews

Good repair ability

It repairs the DNA fragment end, forming a blunt end and phosphorylating the 5′ end of the DNA double-strand.

United States

2022-08-05

Low-temperature transportation

The product is transported in a low-temperature icebox, ensuring its quality.

United States

2023-03-15

Blunt ends

The mix converts DNA containing damaged or incompatible 5′- and/or 3′-protruding ends to 5′-phosphorylated, blunt-ended DNA.

United States

2023-04-06

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High Concentration End-Repair Mix

Product Information

Q & A

Customer Reviews

What is the minimum amount of DNA required when using this enzyme?

Which enzymes are included in this mixture?

What is the reaction buffer?

Good repair ability

Low-temperature transportation

Blunt ends

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