Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EMOT1127
| Cat. No. | EMOT1127 |
| Description | RecA functions in DNA recombination and DNA repair. RecA binds to single stranded DNA, resulting in the polymerization of RecA into a nucleoprotein complex. This complex can align with complementary double stranded DNA, resulting in RecA catalysis of DNA strand exchange. RecA DNA binding is stimulated by ATP hydrolysis or non- hydrolyzable ATP analogs. The RecA-ATP-single stranded DNA complex also can function as a coprotease factor in the proteolytic cleavage of LexA, UmuD and certain bacteriophage proteins. RecA complexed with site-specific oligonucleotides have been used to target and specifically cleave large DNA fragments. |
| Source | A recombinant E. coli strain overexpressing E. coli recA from a plasmid. |
| Concentration | 2.0 mg/mL |
| Size | 1.5 mg |
| Unit Definition | Sold by mass of pure protein determined at OD280 (A280= 0.516 at 1 mg/mL, 1cm). |
| Reaction Buffer | 700 mM Tris-HCl100 mM MgCl250 mM DTTpH 7.6 @ 25 °C |
| Storage | 10 mM Tris-HCl1 mM DTT 0.1 mM EDTA 50% glycerolpH 7.5 at 25 °C |
A: The concentration of the enzyme is 2.0 mg/mL.
A: There are a total of 2 DNA binding sites, where the 1st site forms a complex with single-stranded DNA, and the 2nd site binds to double-stranded DNA to search for homologous sequences.
A: The concentration of the product is determined using the OD 280 method.
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RecA can activate proteases and alter single-stranded DNA molecules.
The success rate is high when using this product for homologous recombination experiments.
After purchasing the reagent, it is delivered to the doorstep, which is very convenient.
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