Mouse Tigit Stable Cell Line-CHO-K1

Name: Mouse Tigit Stable Cell Line-CHO-K1
SKU: CSC-RO0041

Cat.No. : CSC-RO0041 Host Cell: CHO-K1

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Informationn

Cat. No.	CSC-RO0041
Description	This cell line is a stably transfected cell line which expresses Mouse Tigit without any tag.
Gene	Tigit
Gene Species	Mus musculus (Mouse)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Stability	Validated for at least 10 passages
Application	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Shipping	Dry ice
Storage	Liquid nitrogen

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.

Gene Name	Tigit T cell immunoreceptor with Ig and ITIM domains [ Mus musculus ]
Gene Symbol	Tigit
Synonyms	Vstm3; ENSMUSG00000071552
Gene ID	100043314
mRNA Refseq	NM_001146325.1
Protein Refseq	NP_001139797.1
Chromosome Location	16 B4; 16
Function	receptor binding; receptor binding;

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Quick Inquiry

Background

Publications

Q & A

Customer Reviews

TIGIT (also called T cell immunoreceptor with Ig and ITIM domains) is one newly discovered immune receptor on some percentage of T cells and Natural Killer Cells (NK). It is also identified as WUCAM and Vstm3. TIGIT binds to CD155 (PVR) on dendritic cells (DCs), macrophages, etc. with high affinity, and also to CD112 (PVRL2) with lower affinity. Research has shown that TIGIT-Fc fusion proteins interact with PVR on dendritic cells and increase IL-10 secretion, while decreasing IL-12 secretion under LPS stimulation. TIGIT-Fc fusion proteins also inhibit T cell activation in vivo. TIGIT's inhibition of NK cytotoxicity can be blocked by antibodies against its interaction with PVR, which is directed through its ITIM domain.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

First Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *