Human CACNA1A/AP4B1/CACNA2D1/KCNJ2 Stable Cell Line-HEK293

Name: Human CACNA1A/AP4B1/CACNA2D1/KCNJ2 Stable Cell Line-HEK293
SKU: CSC-RI0072
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0072

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0072
Description	This cell line is engineered to overexpress human KCNJ2.
Background	The human CACNA1A gene encodes the pore-forming subunit of Cav2.1, the voltage-gated P/Q-type calcium channel. Inclusion of auxiliary subunits modulate gating and pharmacological characteristics, while the inwardly rectifying potassium channel allows the membrane potential to be altered with changes in extracellular K+. Mutations in CACNA1A are responsible for several inherited neurologic disorders including, familial hemiplegic migraine, episodic ataxia type2, and spinocerebellar ataxia type 6 epilepsy. Cav2.1 channels expressed in neurons are therapeutic targets in pain.
Target Gene	KCNJ2
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuCACNA1A/AP4B1/CACNA2D1/KCNJ2
Alias	KCNJ2, IRK1, Kir2.1, IRK-1, hIRK1, LQT7, SQT3, ATFB9, HHIRK1, KIR2.1, HHBIRK1
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KCNJ2 potassium inwardly-rectifying channel, subfamily J, member 2 [ Homo sapiens ]
Gene Symbol	KCNJ2
Synonyms	IRK1; LQT7; SQT3; ATFB9; HHIRK1; KIR2.1; HHBIRK1
Gene Description	potassium inwardly-rectifying channel, subfamily J, member 2
GeneID	3759
Uni ProtID	P63252
mRNA Refseq	NM_000891.2
Protein Refseq	NP_000882.1
Chromosome Location	17q24.3
Function	identical protein binding; inward rectifier potassium channel activity;
Pathway	Activation of G protein gated Potassium channels, organism-specific biosystem; Activation of GABAB receptors, organism-specific biosystem; Cholinergic synapse, organism-specific biosystem; Classical Kir channels, organism-specific biosystem; G protein gated Potassium channels, organism-specific biosystem; GABA B receptor activation, organism-specific biosystem; GABA receptor activation, organism-specific biosystem;
MIM	600681

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