Cat.No. : CSC-RO1108M Host Cell: HEK293T
| Cat. No. | CSC-RO1108M |
| Description | This cell line is engineered to stably overexpress monkey TREM2-TYROBP (also named as TREM2-DAP12). |
| Gene | TREM2-TYROBP |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The TREM2-TYROBP gene encodes for the Triggering Receptor Expressed on Myeloid Cells 2 (TREM2) and Tyrobor protein (TYROBP), which are proteins involved in the immune response and inflammation. TREM2 is a cell surface receptor expressed on myeloid cells, such as macrophages and microglia, and plays a role in phagocytosis and the clearance of cellular debris and pathogens. TYROBP is a protein that binds to TREM2 and modulates its function.
A: The TREM2-TYROBP gene contributes to immune response and inflammation by enabling myeloid cells to effectively clear pathogens and cellular debris. TREM2 activation leads to the engulfment of microorganisms and dead cells, preventing their accumulation and potential propagation of infection or inflammation. The interaction between TREM2 and TYROBP modulates this process, ensuring proper immune responses and limiting unnecessary inflammation.
A: Mutations in the TREM2-TYROBP gene have been associated with various diseases, including neurodegenerative disorders like Alzheimer's disease and systemic lupus erythematosus. These mutations can lead to alterations in the function of TREM2, affecting the clearance of amyloid plaques and the regulation of inflammation, which may contribute to the pathogenesis of these diseases.
A: The expression of the TREM2-TYROBP gene is regulated by various factors, including cytokines and microbial signals. Activation of TREM2 by pathogens or cellular debris can lead to the upregulation of its expression. Additionally, regulatory mechanisms, such as epigenetic modifications and post-translational modifications, can influence the expression of TREM2 and TYROBP, ensuring proper immune responses and limiting unnecessary inflammation.
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The Monkey TREM2-TYROBP Stable Cell Line-HEK293T is supported by comprehensive off-target detection data, ensuring the specificity of the genetic modifications. The provider employs effective strategies to minimize off-target effects, enhancing the reliability of experimental results.
Detailed background information is provided for the Monkey TREM2-TYROBP Stable Cell Line-HEK293T, including the source of the cell line, the genetic modifications made, and the characterization of the cell line's phenotype. This information is crucial for researchers to understand the context and potential applications of the cell line.
The Monkey TREM2-TYROBP Stable Cell Line-HEK293T has significant clinical relevance, as it can be used to study the function of TREM2 and TYROBP in neurodegenerative diseases, such as Alzheimer's disease.
The Monkey TREM2-TYROBP Stable Cell Line-HEK293T is made available for dissemination and sharing among the scientific community, promoting collaboration and reproducibility of research findings. Researchers can access this cell line through established repositories or directly from the provider, facilitating its widespread use and advancement of scientific knowledge.
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